雌激素和表皮生长因子对成纤维细胞活性的调节作用及机制  

The regulation of Estrogen and epidermal growth factor to fibroblast activity function and mechanism

作　　者：徐路生[1] 李进[1] 杜永军[1] 沈锐[1] 郭凡[2] XU Lu- sheng , LI Jin, DU Yong-jun, SHEN Rui, GUO Fan.(Department of The First People＇s Hospital of Foshan 528000, Chin)

机构地区：[1]佛山市第一人民医院整形美容科,广东佛山528000 [2]佛山市第一人民医院检验科,广东佛山528000

出　　处：《解剖学研究》2018年第1期37-39,48,共4页Anatomy Research

基　　金：佛山市科技局项目(2016AB002741)

摘　　要：探讨在创面皮肤中,雌激素和表皮生长因子对表皮成纤维细胞活性的调节作用及其可能机制。方法分离培养表皮成纤维细胞,分四组,分别对细胞单独和联合应用雌激素、表皮生长因子进行共培养和预处理(10-7mol/L雌激素组、20 ng/ml表皮生长因子组、联合组和对照组),培养24 h、36 h和72 h时检测细胞的生物活性,采用p半乳糖苷酶染色法检测成纤维细胞衰老情况,并采用Western blot检测细胞周期蛋白cyclin D1和凋亡蛋白P53的表达。结果各组细胞共培养24 h后,细胞增殖活性增强,衰老减弱,并且随时间延长而变化明显,具有时间依赖性,其中联合组的变化最为明显,与其他组比较差异具有统计学意义(P<0.05);western blot显示联合组cyclin D1表达增加,P53的表达水平下降最为明显(P<0.05)。结论雌激素和表皮生长因子可调节成纤维细胞的活性,其可能通过下调P53的表达和上调细胞周期蛋白cyclin D1的表达而促进表皮纤维细胞的增殖和减少凋亡。To study the estrogen and epidermal growth factor in the regulation of fibroblast activity in wound skin and its possible mechanism. Methods Fibroblasts cells divided into four groups and were cultivated with estrogen, epider- mal growth factor respectively. After training for 36 h and 24 h, 72 h, testing cell biological activity with MTT and using SA- 8-gal for aging fibroblasts. And cyclinD1 and P53 expression were also be detected with Western blot. Results After cell culture for 24 h, cell proliferation activity increasing and aging decrease too, and changes significantly with the extension of time. This change is most obvious in joint group than the other groups （P 〈 0.05 ）, Western blot showed that increased ex- pression of cyclin DI and decreased expression of P53 in joint group （P 〈 0.05）. Conclusion Estrogen and epidermal growth factor can regulate the activity of the fibroblasts, which may by cutting the expression of P53 and increase the expres- sion of cyclinD 1 to promote proliferation and reduction of apoptosis for fiber cells.

关 键 词：雌激素 表皮生长因子 成纤维细胞 

分 类 号：R622[医药卫生—整形外科]