脐带和脂肪源性间充质干细胞生物学特性比较  

Comparative study on biological characteristics of mesenchymal stem cells from human umbilical cord and adipose

作　　者：黄平[1] 张坤[1,2] 李芳 黄国宝[3] 延冰 肖东杰 汪运山 刘华[1,2] HUANG Ping1 , ZHANG Kun1,2, LI Fang1,2, HUANG Guobao3, YAN Bing4, XIAO Dongjie1,2, WANG Yunshan1,2, LIU Hua1,2(1. Medical Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China; 2. Shandong Research Center of Transplantation and Tissue, Jinan 250013, Shandong, China; 3. Department of Bum and Plastic Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China; 4. Department of Gastrointestinal Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, Chin)

机构地区：[1]山东大学附属济南市中心医院医学实验诊断中心,山东济南250013 [2]山东省移植与组织工程技术研究中心,山东济南250013 [3]山东大学附属济南市中心医院烧伤整复外科,山东济南250013 [4]山东大学附属济南市中心医院胃肠外科,山东济南250013

出　　处：《山东大学学报（医学版）》2018年第3期72-78,共7页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金青年科学基金(81200950);山东省科技发展计划(2014GSF118173);中国博士后科学基金面上项目(2016M590637);山东省医药卫生科技发展计划(2016WS0139;2016WS0134)

摘　　要：目的比较脐带间充质干细胞(UC-MSCs)与脂肪源性间充质干细胞(AD-MSCs)的体外生物学特性。方法分离培养UC-MSCs与AD-MSCs,CCK-8法检测UC-MSCs与AD-MSCs的增殖能力,流式细胞术检测细胞表面标志物表达。油红O,茜红素及Von Kossa染色比较UC-MSCs与AD-MSCs成脂成骨诱导分化能力。MSCs与外周血单个核细胞(PBMC)共培养,在植物血凝素(PHA)刺激作用下,流式细胞术分析MSCs对PBMC增殖抑制作用。结果生长曲线结果显示,UC-MSCs细胞增殖优于AD-MSCs,UC-MSCs与AD-MSCs稳定表达CD13、CD44、CD73及CD90分子,不表达CD34和CD45。AD-M SCs成脂能力优于UC-M SCs,但成骨能力没有明显差异。UC-M SCs抑制PBM C增殖能力优于AD-M SCs。结论 UC-M SCs在免疫调节方面是一种更佳的种子细胞选择。Objective To study the biological characteristics of mesenchymal stem cells（ MSCs） derived from human umbilical cord and adipose in vitro. Methods Umbilical cord-derived mesenchymal stem cells（ UC-MSCs） and adiposederived mesenchymal stem cells（ AD-MSCs） were isolated. Cell growth curve was detected using CCK-8 method,and cell surface antigens were measured using flow cytometry. The adipogenic and osteogenic differentiation assays were analyzed by Oil Red O,Alizarin Red S and Von Kossa staining. The immunomodulatory properties of UC-MSCs and AD-MSCs on lymphocytes were evaluated through the co-culture assay with PHA activated adult peripheral blood monocuclear cells（ PBMCs）. Results Cell growth curve showed that UC-MSCs proliferated more rapidly than AD-MSCs.Flow cytometry analysis showed that UC-MSCs and AD-MSCs were positive for CD13、CD44、CD73 and CD90,andnegative for CD34 and CD45. AD-MSCs had the stronger adipogenetic ability than UC-MSCs,but there were no difference on osteogenetic ability between these two cells. UC-MSCs has the stronger suppression effects on PBMC proliferation than AD-MSCs. Conclusion UC-MSCs are the more attractive cell population for usage in immune cellular therapy.

关 键 词：脐带间充质干细胞 脂肪源性间充质干细胞 细胞增殖 细胞分化 免疫抑制 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]