薯蓣皂苷元对脂多糖诱导的BV2小胶质细胞极化状态的影响  

Impact of diosgenin on the polarization of BV2 microglia induced by lipopolysaccharide

作　　者：王文云 李杰[1] 马铁梁[1] 葛志军[1] 赵彦平[1] WANG Wen-yun,LI Jie,MA Tie-liang,GE Zhi-jun,ZHAO Yan-ping(Affiliated Yixing Hospital of Jiangsu University, Yixing Jiangsu 21420)

机构地区：[1]江苏大学附属宜兴医院,江苏宜兴214200

出　　处：《世界中西医结合杂志》2018年第1期39-43,共5页World Journal of Integrated Traditional and Western Medicine

基　　金：江苏省自然科学基金项目(BK20141121)

摘　　要：目的探讨薯蓣皂苷元(diosgenin)对脂多糖(LPS)诱导下BV2小胶质细胞极化状态的影响。方法将BV2细胞根据不同的处理方法分为对照组,LPS组,LPS+薯蓣皂苷元组。使用MTT法检测BV2细胞的增殖能力,使用ELISA法检测BV2细胞表达肿瘤坏死因子α(TNF-α),白细胞介素10(IL-10)水平,使用实时荧光定量(RT-PCR)法检测BV2细胞IL-10,诱导型一氧化氮合酶(i NOS)以及精氨酸酶(Arg-1)mRNA水平。结果使用不同浓度的薯蓣皂苷元处理BV2细胞后,各浓度的薯蓣皂苷元对BV2细胞的增殖影响无统计学意义(P>0.05),而加入LPS刺激后,发现25μg/ml薯蓣皂苷元为最适浓度,且其最佳作用时间点为24 h(P<0.05);薯蓣皂苷元可以抑制在LPS诱导下BV2细胞TNF-α水平以及i NOS表达,同时提高IL-10水平以及Arg-1表达(P<0.05)。结论薯蓣皂苷元可以通过抑制M1型小胶质细胞极化以及促进M2型小胶质细胞极化进而发挥抗炎的作用。Objective To explore the impact of diosgenin on the polarization state of bv2 microglia induced by lipopolysaccharide（LPS）. Methods According to different treatment methods,BV2 cells were divided into a control group,a LPS group and a LPS plus diosgenin group. MTT method was used to detect the proliferation of BV2. ELISA was adopted for the determination of the expressions of TNF-α and IL-10 in BV2 cells and RT-PCR method was for the determination of the levels of IL-10,i NOS and Arg-1 mRNA.Results After treated with diosgenin of different concentrations,the proliferations of BV2 cells were not different significantly among diosgenin of different concentrations（P〉0. 05）. After stimulated with LPS,it was discovered that the optimal concentration of diosgenin was 25μg/ml and the optimal effect time was 24 h（P〈0. 05）. Diosgenin inhibited the expressions of TNF-α and i NOS and increased the expressions of IL-10 and Arg-1 in BV2 cells induced by LPS（P〈0. 05）. Conclusion Diosgenin plays its action of anti-inflammation through inhibiting the polarization of M1 microglia and promoting the polarization of M2 microglia.

关 键 词：薯蓣皂苷元 BV2小胶质细胞 脂多糖 M1/M2极化状态 炎症 

分 类 号：R285[医药卫生—中药学]