Generation of knock-in cynomolgus monkey via CRISPRJCas9 editing  

出　　处：《Cell Research》2018年第3期379-382,共4页细胞研究（英文版）

摘　　要：Dear Editor, Genetically modified monkey models that exhibit hu- man disease symptoms are versatile tools for studying human disease mechanisms and advancing therapeutic treatments [1]. However, generation of gene-targeted monkeys remains very challenging, due to the long breeding cycle and low gene-editing efficiency [1, 2]. The CRISPR/Cas9 system has been used to generate gene-targeted animals in many species by directly inject- ing Cas9 mRNA and single-guide RNAs （sgRNAs） into pronuclear stage zygotes [3]. Several recent studies have shown that the CRISPR/Cas9 system could also be used to generate gene-knockout monkeys [4-10]. However, no gene knock-in monkey has been reported, presumably because of the low knock-in rate [2].

关 键 词：猴子 编辑 疾病症状 mRNA 繁殖周期 直接注射 治疗学 基因 

分 类 号：S858.31[农业科学—临床兽医学] Q959.848[农业科学—兽医学]