钝顶螺旋藻C-藻蓝蛋白的能量传递动力学  

The energy transfer dynamics in C-phycocyanin hexamer from Spirulina platensis

作　　者：王肖肖 李文军[2] 谢明远 赵福利 杨革[1] 刘琪[2,4] 秦松 Xiaoxiao Wang1,2, Wenjun Li2, Mingyuan Xie3 , Fuli Zhao3, Ge Yangl, Qi Liu2,4 ＆ Song Qin2(1College of Life Science, Qufu Normal University, Qufu 273165, China; 2 gantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China 3 School of Physics, Sun Fat-sen University, Guangzhou 510275, China; 4 School of Pharmacy, Fantai University, Yantai 264005, Chin)

机构地区：[1]曲阜师范大学生命科学学院,曲阜273165 [2]中国科学院烟台海岸带研究所,烟台264003 [3]中山大学物理学院,广州510275 [4]烟台大学药学院,烟台264005

出　　处：《科学通报》2018年第7期641-648,共8页Chinese Science Bulletin

基　　金：国家重点研发项目(2016YFB0601001)资助

摘　　要：结合超快速时间分辨荧光光谱,对钝顶螺旋藻(Spirulina platensis)C-PC六聚体内能量传递过程机制进行了研究.通过不同探测波长下荧光衰减曲线的拟合,对六聚体内能量传递途径和相关传递参数进行了指认,从实验上证实C-藻蓝蛋白六聚体能量传递具有4个时间常数,分别为6,22,280,1470 ps,并对各时间组分进行了指认和讨论.结果反映了部分C-PC中不同发色团之间的能量传递动力学性质,为了解藻胆蛋白的结构和功能的关系提供了重要的实验数据,同时对于理解光合作用的原初过程也有重要的参考价值.Photosynthesis is an ancient and important conversion of solar light to biological energy in photosynthetic organisms. Phycobilisome （PBS） system in cyanobacteria and red algae is one of two major light-harvesting systems of photosynthetic oxygen organisms. PBS is aggregation of water-soluble phycobiliproteins （PBPs） which are distinctively colored group of disk-shaped macromolecular proteins bearing covalently attached open-chain tetrapyrroles known as phycobilins. PBPs are capable to absorb solar light and transfer energy to the chlorophyll of photosynthetic reaction center with a high efficiency. Light energy absorbed by phycobilins is transmitted between the subunits, and then transmitted between different PBPs, finally to the photoreaction center located on the thylakoid membrane. The energy transfer between PBPs is very fast and high efficiency. Because energy can be transferred in different chromophores and there are a large number of chromophores in PBPs, the energy transfer path of PBPs is complex and diverse. In the past 20 years, energy transfer process of PBPs have been analyzed by time-resolved fluorescence spectroscopy because a large shift of the fluorescence peaks can be clearly observed. For example, Zhang et al. estimated the time constant for energy transfer from PC （rod） to APC （core） as 18 ps and 55 ps based on PBPs crystal structures and time-resolved fluorescence spectra of the rod-core complex. However, due to the technical bottleneck of theoretical simulation and spectral experimental detection, the research on the physical mechanism of photosynthetic energy transfer is relatively slow. In recent years, the ultrafast spectrum time resolution has been continuously improved, which provides the conditions for studying the energy transfer of PBPs. In this paper, the mechanism of energy transfer in C-phycocyanin hexamer from Spirulina platensis was studied by ultrafast time-resolved fluorescence spectroscopy. The energy transfer pathway and the related transfer parameters in th

关 键 词：C-藻蓝蛋白六聚体 时间分辨光谱 能量传递 

分 类 号：Q946.1[生物学—植物学]