紫花苜蓿MsSAG113基因的克隆及对拟南芥的转化  

Cloning of MsSAG113 Gene From Medicago sativa L. and Transformation of Arabidopsis thaliana

作　　者：宿强 曾会明[1] 许立新[1] 晁跃辉[1] XU Qiang, ZENG H uiming, XU Lixin, CHAO Yuehui(College of Forestry,Beijing Forestry University,Beijing 10008)

机构地区：[1]北京林业大学林学院,北京100083

出　　处：《北方园艺》2018年第6期10-15,共6页Northern Horticulture

基　　金：北京林业大学科技创新计划资助项目(2017ZY16)

摘　　要：以紫花苜蓿为试材,采用RT-PCR技术克隆出一个与拟南芥SAG113同源的基因,利用无缝连接酶将其与3302Y连接构建过量表达的植物表达载体,通过花序浸泡法转化拟南芥获得具有草铵膦抗性的植株,以期为研究MsSAG113的功能提供参考依据。结果表明:MsSAG113基因编码区长849bp,编码283个氨基酸,PCR和RT-PCR检测显示成功获得了转MsSAG113基因拟南芥植株,初步证明在转基因拟南芥中外源SAG113基因能够转录表达。Alfalfa was used as material to clone a homologous gene named SAG113 in Arabidopsis thaliana by RT-PCR technique.The MsSAG113 gene was inserted into plasmid 3302 Y with seamless ligase constructing a plant expressing vector,and transgenic Arabidopsis thaliana plants with glufosinate-ammonium resistance were screened by floral dip method.The transgenic materials provide the foundation for further study of MsSAG113 gene.The results showed that its coding domain sequence was 849 bp in length encoding 283 amino acids,and PCR and RT-PCR analysis confirmed the transgenic Arabidopsis thaliana plants with MsSAG113 gene were successfully obtained,which preliminary proved transcription and expression of exogenous gene SAG113 in transgenic Arabidopsis thaliana was able.

关 键 词：紫花苜蓿 SAG113基因 拟南芥 转化 

分 类 号：S551.7[农业科学—作物学]