甜叶菊叶片外植体再生体系的建立  

Establishment of a Regeneration System From Leaf Explant of Stevia rebaudiana

作　　者：包晗 张芮[1,2] 张美玲 张虹[1,2] 陈任 BAO Han1,2, ZHANG Rui1,2, ZHANG Meiling1,2, ZHANG Hong1,2 ,CHEN Ren1,2(1. Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University,Yinchuan,Ningxia 750021; 2. School of Life Science,Ningxia University, Yinchuan,Ningxia 75002)

机构地区：[1]宁夏大学西部特色生物资源保护与利用教育部重点实验室,宁夏银川750021 [2]宁夏大学生命科学学院,宁夏银川750021

出　　处：《北方园艺》2018年第6期16-22,共7页Northern Horticulture

基　　金：宁夏大学引进人才科研启动经费资助项目(BQC2012001)

摘　　要：以甜叶菊品种"中山二号"为试材,首先选用叶片、茎段和茎节3种外植体,在添加3种植物激素萘乙酸(1-Naphthaleneacetic acid,NAA)、苄氨基嘌呤(6-Benzylaminopurine,BAP)和激动素(kinetin,KT)不同浓度组合的MS(murashige and skoog)培养基上培养,明确其对愈伤组织诱导和不定芽分化的影响。以叶片作为外植体,使用激素噻苯隆(Nphenyl-N′^(1),2,3-thidiazol-5-yl-urea,TDZ)替换NAA(BAP和KT浓度范围不变),在不同浓度组合的MS培养基上进行愈伤组织、不定芽和丛生芽诱导,以期建立一套快速、有效的甜叶菊再生体系。结果表明:在培养基中TDZ浓度达到3.0mg·L^(1)、BAP 1.0mg·L^(1)和KT0.5mg·L^(1)时,诱导的愈伤组织分化的不定芽最多,且随后形成了大量的丛生芽。丛生芽继代到无添加激素的1/2MS培养基上,能够继续生长,分化成茎叶和根,形成完整植株。该再生体系生产周期短、繁殖系数高,可用于生产上大规模快速繁殖和基因转化研究。In order to develop a rapid and efficient method for plant regeneration of Steviarebaudiana,the segments of leaf,stem and nodalcut from the cultivar‘Zhongshan No.2＇,were used as explants,and the effects of the MS（murashige and skoog）basal medium supplied with three kinds of plant growth regulators,NAA（1-Naphthaleneacetic acid）,BAP（6-Benzylaminopurine）and KT（kinetin）in different concentrations on the stages of callus and adventitious bud inducement were determined.Then the leaf was selected as optimal explant,and was cultured on the media that substituted TDZ（Nphenyl-N′^（1）,2,3-thidiazol-5-yl-urea）for NAA（BAP and KT remained unchanged）.The results showed that the most adventitious buds were differentiated from the induced calli on the MS basal medium supplied with 3.0 mg·L^（1） TDZ combined with 1.0 mg·L^（1） BAP and 0.5 mg·L^（1） KT,following a large number of cluster bud sappeared.The cluster buds elongated and differentiated leaves,stem and roots into a complete plantlet.With short production cycle,high propagation coefficient,the regeneration method could be used for rapid mass propagation and gene transformation.

关 键 词：甜叶菊 组织培养 植物激素 愈伤组织 不定芽 丛生芽 

分 类 号：S566.903.51[农业科学—作物学]