向日葵核盘菌菌饼打取位置以及继代次数对其生物学特性和致病力的影响  

Influence of mycelium plug sampling points and subculture times on biological characteristics and pathogenicity of sunflower Sclerotinia sclerotorium

作　　者：于悦[1] 卜浩宇 贾瑞芳 孟庆林 张键[2] Yu Yue1, Bu Haoyu2, Jia Ruifang2, Meng Qinglin3, Zhang Jian2(1. Beijing Forestry University, Beijing 100053 China; 2. Inner Mongolia Agricultural University, Hohhot Inner Mongolia 010018, China; 3. Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences, Harbin Heilongjiang 150086, Chin)

机构地区：[1]北京林业大学林学院,北京100083 [2]内蒙古农业大学农学院,内蒙古呼和浩特010018 [3]黑龙江农业科学院植物保护研究所,黑龙江哈尔滨150086

出　　处：《中国植保导刊》2018年第3期5-11,共7页China Plant Protection

摘　　要：在向日葵核盘菌培养中,采用不同继代次数和打取不同位置的菌饼对其生物学特性和致病力的影响进行了研究。结果表明,在距离接种中心最远处4.5cm处打取的核盘菌菌饼,其生长速率最快,草酸分泌量最高(94.54μg/mg),多聚半乳糖醛酸酶活性最高(29.15 U/mg),且致病力最强(病斑直径1.79 cm)。同时,通过比较核盘菌培养继代次数对致病力的影响,发现当继代次数超过15次后,其致病力显著降低。In this study, we analyze the influence of sub-culture frequency and distance of mycelium plug sampling positions related to inoculated center on biological characteristics and pathogenicity of sunflower Sclerotinia sclerotorium. The results indicated that with sampling point at 4.5 cm far from the inoculated center, the growth of mycelium was fastest （3.61cm）, OA level and PG enzymes activity were the highest （94.53μg/mg, 29.15 U/mg, respectively） and virulence on the inoculated host was strongest （with lesion size of 1.79 cm）. Based on comparing of the effect between different subculture times, it was discovered that the pathogenicity reduced significantly after 15 times of sub-culture frequency.

关 键 词：向日葵核盘菌 菌饼打取位置 继代次数 致病力 

分 类 号：S435.2[农业科学—农业昆虫与害虫防治]