辐射诱变结合抗自身产物选育替考拉宁高产菌株  

Screening for high teicoplanin-producing strain by mutation combined with resistance to self-produced metabolite

作　　者：张引[1] 张祝兰[1] 邱观荣[1] 杨煌建[1] 王德森[1] 任林英[1] 连云阳[1] ZHANG Yin ZHANG Zhulan QIU Guanrong YANG Huangjian WANG Desen REN Linying LIAN Ytmyang(Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou 350007, Chin)

机构地区：[1]福建省微生物研究所福建省新药(微生物)筛选重点实验室,福州350007

出　　处：《辐射研究与辐射工艺学报》2018年第1期43-48,共6页Journal of Radiation Research and Radiation Processing

基　　金：福建省科技重大项目(2014YZ0004-2);福建省化药技术重大研发平台(2014Y2001);福州市生物制药行业技术创新中心(2016-PT-36);福建省公益类科研院所专项(2017R1009-13)资助~~

摘　　要：采用^(60)Co γ-射线辐射结合抗性筛选技术,对替考拉宁产生菌替考游动放线菌FIM-16的孢子进行诱变处理,以替考拉宁自身抗性作为选择压力,并通过摇瓶发酵对抗性基因突变株进行筛选。结果获得一株摇瓶发酵水平较出发菌株FIM-16提高两倍的高产菌FIM-16-58,且遗传性状相对稳定,菌种传3代发酵水平无明显影响,菌种斜面在4°C稳定保藏两个月;其在1 T发酵罐上发酵,添加0.1%的玉米油可有效抑制泡沫的产生,替考拉宁产量达5.0 g/L以上。To screen high-yield teicoplanin-producing strain, ^（60）Co γ-ray irradiated spore suspension of the strain Actinoplanes teichomyceticus FIM-16 was spread on regenerating plate containing lethal concentration of teicoplanin. Teicoplanin-resistant mutants were picked and screened by shake-flask fermentation. Strain FIM-16-58 showed two times higher teicoplanin yield than the original strain, and demonstrated higher hereditary stability. There was no obvious effect on the fermentation level of the strain over three generations, and its slope was stable at 4 °C for two months. During scale-up experiment in the 1 T fermenter, foam was suppressed effectively by adding 0.1% corn oil, and the yield of teicoplanin reached over 5.0 g/L. Therefore, the high-yield strain laid the foundation of its industrial production and fermentation.

关 键 词：替考游动放线菌 抗性突变筛选 辐射诱变 替考拉宁 

分 类 号：TL99[核科学技术—核技术及应用] TQ465.92[化学工程—制药化工]