龟甲对糖皮质激素性骨质疏松大鼠腰椎和胫骨骨密度、骨强度的影响  

Effect of Tortoiseshell on Bone Mineral Density and Bone Strength of Lumbar Vertebrae and Tibia in Glucocorticoid-induced Osteoporosis Rats

作　　者：张志达[1] 任辉[2] 沈耿杨[1] 梁德[2] 杨志东[2] 黄锦菁[1] 招文华 余佩沅 尚奇[1] 江晓兵[2] ZHANG Zhida1, REN Hui2, SHEN Gengyang1 , LIANG De2, YANG Zhidong2, HUANG Jinjing1 , ZHAO Wenhua1, YU Peiyuan1, SHANG Qi1 , JIANG Xiaobing2(1. Guangzhou University of Chinese Medicine, Guangzhou, 510405 ; 2. First Affiliated Hospital of Guangzhou University of Chinese Medicin)

机构地区：[1]广州中医药大学,广东省广州市机场路12号510405 [2]广州中医药大学第一附属医院

出　　处：《中医杂志》2018年第7期617-620,共4页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81774338;81503591);广东省自然科学基金(2016A030313645;2014A030310082);广东省省级科技计划(2014A020221021);广州中医药大学第一临床医学院优秀博士学位论文培育项目(YB201602)

摘　　要：目的观察龟甲对糖皮质激素性骨质疏松大鼠腰椎、胫骨骨密度和骨强度的作用差异。方法 48只SD大鼠随机分为空白组、模型组、西药组和龟甲组,每组12只。模型组、西药组和龟甲组皮下注射地塞米松制造骨质疏松模型。造模后西药组用阿仑膦酸钠63 mg/kg灌胃,每7天1次;龟甲组用龟甲30 mg/(kg·d)灌胃,每天1次;空白组和模型组用0.9%氯化钠溶液2 ml灌胃,每天1次。各组均给药3个月。分别于给药前及给药2个月、3个月检测各组大鼠腰椎、胫骨骨密度和骨强度,计算给药3个月时西药组与龟甲组骨密度及骨强度改善率。结果龟甲组给药2、3个月腰椎、胫骨骨密度,胫骨骨强度均升高,给药3个月腰椎骨强度亦升高(P<0.05或P<0.01)。给药3个月时,西药组、龟甲组腰椎骨密度、骨强度及胫骨骨密度、骨强度均高于模型组(P<0.05或P<0.01);龟甲组腰椎骨密度低于西药组(P<0.05),而腰椎骨强度及胫骨骨密度、骨强度与西药组比较差异均无统计学意义(P>0.05)。给药3个月后,龟甲组腰椎骨密度改善率为8.08%,胫骨骨密度改善率为8.30%,腰椎骨强度改善率为44.67%,胫骨骨强度改善率为30.58%。结论龟甲对糖皮质激素性骨质疏松大鼠腰椎、胫骨骨密度和骨强度均有改善作用,且对腰椎骨强度改善作用优于胫骨,对二者骨密度改善作用相当。Objective To observe the effect of tortoiseshell on the bone mineral density（BMD） and bone strength of lumbar vertebrae and tibia in glucocorticoid-induced osteoporosis rats. Methods Forty-eight SD rats were randomized into blank group,model group,western medicine group and tortoiseshell group,12 rats in each group. Dexamethasone was injected subcutaneously to make the osteoporosis model in the model group,western medicine group,and tortoiseshell group. After modeling,the western medicine group was treated with alendronate sodium63 mg/kg orally once every 7 days; the tortoiseshell group was treated with tortoiseshell 30 mg/（kg ·d） by gavage once a day; the blank group and the model group were treated with 0. 9% chlorine sodium solution 2 ml by gavage once a day. All groups were administered for 3 months. The BMD and bone strength of lumbar vertebrae and tibias were measured before treatment,2 months and 3 months after administration. The BMD and bone strength improvement rate of western medicine group and tortoiseshell group were calculated 3 months after administration. Results In the tortoiseshell group,the BMD of lumbar vertebrae and tibia increased 2 and 3 months after administration,so did the strength of tibia; the intensity of lumbar vertebrae also increased 3 months after administration（P〈0. 05 or P〈0. 01）. Three months after administration,the BMD and bone strength of lumber vertebrae and tibia in the western medicine group and the tortoiseshell group were all higher than those in the model group（P〈0. 05 or P〈0. 01）.The BMD of lumbar vertebrae was lower in the tortoiseshell group than in the western medicine group（P〈0. 05）,but there was no significant difference in the lumbar vertebrae bone strength and tibia BMD and bone strength（P〈0. 05）. After 3 months of administration,in the tortoiseshell group,the BMD improvement rate of lumber vertebrae was 8. 08%,of tibia 8. 30%,the strength improvement rate of the lumber vertebrae was 44. 67%,of tibia 30. 58%.Conclusion The t

关 键 词：糖皮质激素性骨质疏松 龟甲 骨密度 骨强度 

分 类 号：R285.5[医药卫生—中药学]