七氟醚后处理对糖尿病大鼠脑缺血-再灌注损伤的影响  

Effects of sevoflurane post treatment on cerebral ischemia-reperfusion injury in diabetic rats and non-diabetic rats

作　　者：刘振国[1] 董桂敏[1] 张海燕[1] 刘伟亮[1] LIU Zhenguo , DONG Guimin , ZHANG Haiyan , LIU Weiliang.(Weifang People＇s Hos- pital , Weifang 261000, Chin)

机构地区：[1]山东省潍坊市人民医院麻醉科,261000

出　　处：《临床麻醉学杂志》2018年第2期171-174,共4页Journal of Clinical Anesthesiology

基　　金：潍坊市科学技术发展计划项目(2015WS084)

摘　　要：目的研究七氟醚后处理对糖尿病大鼠脑缺血-再灌注损伤的影响。方法雄性SD大鼠32只,3.0~3.5月龄,体重280~320 g,采用随机数字表法分为四组:非糖尿病缺血-再灌注对照组(NDC组)、糖尿病缺血-再灌注对照组(DC组)、非糖尿病缺血-再灌注七氟醚后处理组(NDS组)和糖尿病缺血-再灌注七氟醚后处理组(DS组),每组8只。采用栓线法和链脲佐霉素制备缺血-再灌注损伤大鼠模型和糖尿病大鼠模型。缺血2 h,再灌注24 h后,进行大鼠神经缺损评分,采用TTC法测定脑梗死体积,Western blot法测定血管生成素-1(angiopoiettin-1,Ang-1)蛋白含量。结果DC组神经缺损评分、脑梗死体积百分比明显高于NDC组,Ang-1蛋白含量明显低于NDC组(P<0.05);NDS组神经缺损评分、脑梗死体积百分比明显低于NDC组,Ang-1蛋白含量明显高于NDC组(P<0.05);DS组神经缺损评分、脑梗死体积百分比明显低于DC组,Ang-1蛋白含量明显高于DC组(P<0.05)。结论大鼠脑缺血-再灌注后,糖尿病可加重神经缺损,增大脑梗死体积,减少Ang-1蛋白含量;七氟醚可减轻神经缺损,减小脑梗死体积,增加Ang-1蛋白含量;七氟醚后处理减轻大鼠脑缺血-再灌注损伤与Ang-1蛋白含量增加有关。Objective To investigate the effects of sevoflurane post treatment on cerebral ische- mia-reperfusion injury in diabetic rats and non-diabetic rats. Methods Thirty-two male SD rats, aged 3.0-3.5 months, weighing 280-320 g, were divided into four groups by random number method （n = 8）： non diabetic ischemia reperfusion group （group NDC）; diabetic isehemia reperfusion group （group DC）; non diabetic ischemia reperfusion group with the sevoflurane post-treatment （group NDS） ; diabetic ischemia reperfusion group with the sevofturane post-treatment （group DS）. The mid- dle cerebral artery occlusion method and streptozotocin were used to establish the ischemia- reperfusion injury model and diabetic model. 2 h after ischemia and 24 h after reperfusion, the rats nerve defect scale was detected, the cerebral infarction volume was evaluated by TTC method, and Western blot method was used to detect angiogenin-1 （Ang-l） expression. Results Between the four groups of rats, nerve deficit score and infarct volume were significantly higher, Ang-1 protein relative expression was significantly lower in group DC than those in group NDC （P〈0. 05） ; neural deficit score and infarct volume were significantly lower, Ang-1 protein relative expression was significantly higher in group NDS than those in group NIX; （P〈0.05） ; nerve deficit score and infarct volume were significantly lower, Ang-1 protein relative expression was significantly higher in group DS than those in group DC （P〈0. 05）. Conclusion In the rats after cerebral ischemia reperfusion, diabetes could aggravate the nerve defect, increase the volume of cerebral infarction, reduce the expression of Ang-1; sevoflurane could reduce nerve defects, reduce infarct volume, increase the expression of Ang-1. The expression of Ang-1 and degree of injury after cerebral ischemia reperfusion in rats have relationship.

关 键 词：七氟醚 糖尿病 缺血-再灌注损伤 血管生成素-1 

分 类 号：R614[医药卫生—麻醉学]