1,2-二氯乙烷对NIH小鼠学习记忆的影响  

Effect of 1,2-dichloroethane on learning and memory in NIH mice

作　　者：张雅婷 钟怡洲 李国樑[2] 郑杰蔚 吴洁姣 曾丽海[2] 黄曼琪 谢植伟[2] 陆丰荣[2] 梁博萱 江亮 郑倩玲[2] 黄振烈 ZHANG Yating , ZHONG Yizhou, LI Guoliang, ZHENG Jiewei, WU Jiejiao, ZENG Lihai, HUANG Manqi, XIE Zhiwei, LU Fengrong, LIANG Boxuan, JIANG Liang, ZHENG Qianling, HUANG Zhenlie(School of Public Health, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510315, Chin)

机构地区：[1]广东药科大学公共卫生学院,广东广州510315 [2]广东省职业病防治院,广东省职业病防治重点实验室,510300 [3]中山大学公共卫生学院,510275

出　　处：《中国职业医学》2018年第1期1-6,共6页China Occupational Medicine

基　　金：国家自然科学基金(81202245);国家临床重点专科建设项目(2011-09);广东省医学科学技术研究基金(A2016137;A2016557;A2017193);广州市科技计划项目(201707010405);广东省职业病防治重点实验室开放运行(2017年度考核合格)(2017B030314152);广东省化学中毒与核辐射突发事件医学救援应急技术研究中心(2012A032100002)

摘　　要：目的研究1,2-二氯乙烷(1,2-DCE)亚急性全身动式吸入暴露对NIH小鼠学习记忆能力的影响。方法无特定病原体级健康7周龄NIH小鼠45只,随机分为对照组、低剂量组和高剂量组,每组雌鼠5只,雄鼠10只。采用全身动式吸入染毒方式,分别予剂量为0.00、100.00、350.00 mg/m3的1,2-DCE染毒,6 h/d,连续28 d。分别于染毒前和染毒第1~4周采用Morris水迷宫实验对NIH小鼠进行神经行为学测试。结果 3组小鼠的体质量和游泳速度分别比较,差异均无统计学意义(P>0.05)。定位航行实验结果显示,染毒第1~4周,低和高剂量组小鼠的逃避潜伏期均较同时间点对照组延长(P<0.05);对照组小鼠染毒第1~4周的逃避潜伏期均较同组染毒前缩短(P<0.05);而高剂量组小鼠逃避潜伏期随染毒时间增加而延长,在染毒第4周逃避潜伏期较同组染毒前出现有统计学意义的延长(P<0.05)。空间探索实验结果显示,低和高剂量组小鼠染毒第2~4周的第1次穿越平台时间均较同时间点对照组延长(P<0.05);低和高剂量组小鼠的目标象限停留时间和穿越平台次数均低于对照组(P<0.05)。结论 1,2-DCE亚急性吸入暴露可致NIH小鼠的学习记忆能力损伤;高剂量染毒时可致学习能力呈时间-效应性降低。Objective To investigate the effects of subacute systemic inhalation exposure of 1, 2-dichloroethane （1,2-DCE） on learning and memory in NIH mice. Methods Forty-five specific pathogen free healthy 7-week-old NIH mice were randomly divided into control, low-dose and high-dose groups with 5 female mice and 10 male mice in each group. The mice were exposed to 1,2-DCE at dosages of 0. 00, 100. 00 and 350. 00 mg/m3 for 6 hours per day for consecutive 28 days by dynamic systemic inhalation. The neurobehavioral tests of mice were performed before and after the first to fourth weeks of exposure using the Morris water maze test. Results There was no significant difference in body weight and swimming speed among the three groups of mice （ P 〉 0. 05 ）. The navigation experiment results showed that the escape latency of mice in both low- and high-dose groups were longer than that of the control group at the same time point （P 〈0. 05） during 1-4 weeks after exposure. In the control group, the escape latency was shorter than that of the same group before exposure （P 〈 0. 05）. The escape latency of high-dose group prolonged with the increase of exposure time, and in the 4th week the escape latency was significantly higher than that of the same group before exposure （ P 〈 0. 05 ）. The experiment results of space exploration indicated that the first time of crossing platform in low- and high-dose groups were longer than that of the control group at the second to the fourth week （ P 〈 0. 05 ）. The target quadrant retention time and the number of crossing the platform in the low- and high-dose groups were lower than those in the control group （P 〈 0. 05）. Conclusion Subacute inhalation exposure of 1,2-DCE can impair the learning and memory ability of NIH mice.The high-dose exposure may reduce learning ability in mice in a time-effect manner.

关 键 词：1 2-二氯乙烷 亚急性 动式吸入 MORRIS水迷宫实验 学习记忆 神经行为 小鼠 

分 类 号：R114[医药卫生—卫生毒理学]