长链非编码RNA在桥本甲状腺炎合并甲状腺乳头状癌中的表达研究  

Expression of long noncoding RNA in papillary thyroid carcinoma with Hashimoto＇s thyroiditis

作　　者：张煜[1] 丁金旺[1] 罗定存[1] 彭友[1] 张卧[1] 潘钢[1] 余道军[2] Zhang Yu, Ding Jinwang, Luo Dingcun, Peng You, Zhang Wo, Pan Gang, Yu Daojun(1.Department of Oncological Surgery, Hangzhou First People＇s Hospital, Nanjing Medical University, Hangzhou 310006, China; 2.Department of Gene Diagnosis Labora- tory, Hangzhou First People＇s Hospital, Nanjing Medical University, Hangzhou 310006, Chin)

机构地区：[1]浙江省杭州市第一人民医院肿瘤外科,杭州市310006 [2]浙江省杭州市第一人民医院基因实验室,杭州市310006

出　　处：《中华内分泌外科杂志》2018年第1期24-29,共6页Chinese Journal of Endocrine Surgery

基　　金：杭州市医药卫生科技计划（重点）项目（2013204）;杭州市重大科技创新专项项目（20131813A08）;浙江省杭州市医药卫生科技计划项目（20128004）;浙江省公益技术应用研究项目（2017C33180）

摘　　要：目的探讨及分析长链非编码RNAs（lncRNAs）在PTC合并桥本甲状腺炎（hashimoto’s thy-roiditis，HT）及不合并HT组织中的表达差异。方法选取55例甲状腺手术组织标本，利用高通量lncRNA基因芯片检测PTC合并HT（A组）及不合并HT（B组）之间lncRNAs的表达谱差异，并通过QRT-PCR进行验证。结果芯片共筛选出1 031条差异表达的IncRNAs和1 388条mRNA；GO分析显示了免疫系统进程、免疫反应等生物学过程发生了明显变化；QRT-PCR验证发现uc002stn.1、ENST00000452578及uc002sti.1这3个lncRNAs在A组和B组中表达存在明显差异。结论PTC合并HT与不合并HT中存在明显的IncRNAs差异表达，且这些lncRNAs及某些生物学过程可能在HT恶变过程中发挥重要作用。Objective To analyze long noncoding RNAs （lncRNAs） expression profiles in papillary thyroid carcinoma （PTC） with Hashimoto＇s thyroiditis （PTC-HT, group A） and PTC only （PTC, group B）. Methods 55 cases of thyroid species were collected. High-throughput microarray lncRNA was used to detect the expression difference of lncRNAs between group A and group B. Real-time quantitative PCR （QRT-PCR） was used to verify. Results 1031 lncRNAs and 1338 mRNAs were detected abnormally expressed in tissue samples of group A compared to B. GO and Pathway analysis of mRNAs suggested some biological processes changed obviously, such as immune system and immune reaction. QRT-PCR showed that the expression of uc002stn.1, ENST00000452578 and ue002sti.1 in group A and group B was significantly different. Conclusion IncRNAs expression was significantly different in PTC with or without HT, which may play important roles in the pathogenesis of PTC with HT.

关 键 词：桥本甲状腺炎 甲状腺乳头状癌 长链非编码RNA 基因芯片 

分 类 号：R581.4[医药卫生—内分泌]