Hcy通过下调VEGF表达而抑制HUVEC的活性及迁移能力  

Hcy inhibits the activity and mobility of HUVEC by lowering VEGF expression

作　　者：于剑 李光新[1] 王坤[1] 王宾[1] 王辉[1] 史作磊[1] 于振海[1] Yu Jian, Li Guangxin, Wang Kun ,Wang Bin ,Wang Hui, Shi Zuolei, Yu Zhenhai(Department of Vascular Surgery, Qianfoshan Hospital of Shandong Province, Jinan 250014, Chin)

机构地区：[1]山东省千佛山医院血管外二科,济南250014

出　　处：《血管与腔内血管外科杂志》2017年第5期955-958,961,共5页Journal of Vascular and Endovascular Surgery

摘　　要：目的探究同型半胱氨酸(Hcy)对脐静脉内皮细胞(HUVEC)的活性及迁移能力的影响及其相应的作用机制。方法检测收集的临床标本中Hcy表达量与下肢深静脉血栓形成(DVT)的关系,利用CCK8及Transwell实验检测Hcy对HUVEC活性及迁移能力的影响,并进一步检测其血管内皮生长因子(VEGF)的表达,验证VEGF对Hcy作用的影响。结果 DVT患者血清Hcy浓度显著增高,体外实验证实Hcy可以显著抑制HUVEC的活性及迁移能力,并可以下调其VEGF的表达,且Hcy对HUVEC活性及迁移能力的抑制作用是通过下调VEGF的表达而实现的。结论 Hcy可通过下调VEGF的表达而抑制内皮细胞的活性及迁移能力,这可能是高Hcy血症的患者容易发生DVT的原因。Objective To explore the effect of homocysteine（Hcy） on the activity and mobility of umbilical vein endothelial cells（HUVEC） and its corresponding mechanism. Methods The relationship between Hcy expression and deep vein thrombosis（DVT） in the collected clinical specimens was detected, the effects of Hcy on HUVEC activity and migration ability were detected by CCK8 and Transwell test, and the expression of VEGF was further tested to verify the effect of VEGF on Hcy. Results The concentration of serum Hcy in DVT patients increased significantly; in vitro experiments,it was demonstrated that Hcy can significantly inhibit the activity and migration of HUVEC, and can reduce the expression of VEGF. The inhibitory effect of Hcy on HUVEC activity and migration ability was achieved by lowering VEGF expression. Conclusion Hcy can inhibit the activity and migration of endothelial cells by lowering the expression of VEGF, which may be the reason of DVT in patients with high Hcy.

关 键 词：同型半胱氨酸 下肢深静脉血栓形成 脐静脉内皮细胞 血管内皮生长因子 

分 类 号：R541.4[医药卫生—心血管疾病]