不同抗性甜瓜接种蔓枯病菌后内源激素含量变化及其相关基因的表达分析  

Changes of endogenous hormone contents and expression analysis of related genes in melon with different resistance after inoculated with Didymella bryoniae

作　　者：鲁秀梅[1] 张宁[1] 夏美玲[1] 钱春桃[1] 陈劲枫[1] LU Xiumei, ZHANG Ning, XIA Meiling, QIAN Chuntao, CHEN Jinfeng(College of Horticulture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Chin)

机构地区：[1]南京农业大学园艺学院/作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《南京农业大学学报》2018年第2期248-255,共8页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金新疆联合基金项目(UI178307)

摘　　要：[目的]本文旨在研究甜瓜抗感材料中内源激素含量及其相关基因表达量的分析,了解甜瓜抗蔓枯病的抗性机制。[方法]以抗蔓枯病甜瓜抗源PI420145和感蔓枯病甜瓜‘白皮脆’(对照)为材料,在接种蔓枯病菌后的不同时间(0、1、2、3、5、7 d)进行取样,测定内源激素乙烯、茉莉酸及水杨酸的含量,并利用荧光定量PCR技术分析叶片中乙烯、茉莉酸、水杨酸等防御信号途径中相关基因的表达特征。[结果]抗蔓枯病甜瓜材料PI420145叶片中的乙烯含量整体低于感蔓枯病材料‘白皮脆’,但在接种0和7 d时茉莉酸含量均高于‘白皮脆’,并且水杨酸含量也基本高于‘白皮脆’。在接种前期(0~3 d),PI420145叶片中乙烯途径相关基因ACS、EIN2的表达量显著高于‘白皮脆’,接种后期(5~7 d)ACS在PI420145中下调表达并且表达量显著低于‘白皮脆’。PI420145叶片中茉莉酸途径相关基因COI1、PDF1.2的表达量分别在接种后期和接种前期基本显著高于‘白皮脆’,而AOS表达量在接种后期则极显著低于‘白皮脆’。在PI420145叶片中除接种后1 d外,水杨酸途径相关基因EDS5的表达量均显著高于‘白皮脆’,而ICS、NPR1的表达量分别在接种后期和接种前期基本显著高于‘白皮脆’。[结论]PI420145叶片中低浓度的乙烯以及高浓度的茉莉酸和水杨酸提高了其对蔓枯病的抗病能力,PI420145叶片中EIN2、PDF1.2、EDS5和NPR1在接种早期的高表达促使其比‘白皮脆’更早一步对蔓枯病菌的侵染作出反应。[Objectives]The article aims to study the endogenous hormone and expression of related genes in melon with different resistance to gummy stem blight after being inoculated with Didymella bryoniae,and to understand the resistance mechanism of melon to gummy stem blight.[Methods]To analyze the changes of ethylene（ETH）,jasmonic acid（JA）,salicylic acid（SA） and expression levels of related genes in melon with different resistance after being inoculated with Didynella bryoniae,leaves of resistant resource PI420145 and susceptible‘Baipicui＇were sampled for real-time PCR and determination of endogenous hormone after inoculation 0,1,2,3,5 and 7 d.[Results]ETH content in PI420145 was lower than that in‘Baipicui＇.However,JA content in PI420145 was higher than that in‘Baipicui＇at 0 and 7 d,and SA content in PI420145 was mainly higher than that in‘Baipicui＇.In the early days of inoculation（0-3 d）,the expression levels of ACS and EIN2 in PI420145 were significantly higher than those in‘Baipicui＇.In the later period of inoculation（5-7 d）,ACS was down-regulated and the expression level in PI420145 was significantly lower than that in‘Baipicui＇.The expression levels of COI1 and PDF1.2 in PI420145 were mainly significantly higher than those in‘Baipicui＇in the early days of inoculation and in the later period of inoculation respectively.However,the expression level of AOS in PI420145 was significantly lower than that in‘Baipicui＇in the later period of inoculation.The expression levels of EDS5 were significantly higher than those in‘Baipicui＇except 1 d after inoculation,and the expression levels of ICS and NPR1 were mainly significantly higher than those in‘Baipicui＇in the early days of inoculation and in the later period of inoculation respectively.[Conclusions]Low concentration ETH and high concentration JA and SA in PI420145 enhanced their resistance to gummy stem blight.The high expression level of EIN2,PDF1.2,EDS5 and NPR1 in PI420145 in the early days of inoculation m

关 键 词：甜瓜 蔓枯病 内源激素 基因表达 

分 类 号：S652.9[农业科学—果树学]