穿心莲提取物抗柯萨奇病毒A16型感染的体外研究  

Effect of Andrographis paniculata Nees extract on coxsackievirus A16 in vitro

作　　者：姜慧芬[1] 张丽红[2] 高丽美[3] 徐笑红[1] 郑晓妍[1] 罗永能[3] Jiang Huifen, Zhang Lihong, Gao Limei, Xu Xiaohong, Zheng Xiaoyan, Luo Yongneng(Clinical Laboratory, Zhejiang Cancer Hospital, Hangzhou 310022, China （ Jiang HF, Xu XH, Zheng XY） ; Clinical Laboratory Center, Shaoxing People＇ s Hospital, Shaoxing 312000, Zhejiang Province, China （ Zhang LH） ; Institute of Viral Diseases, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China （Gao LM, Luo YN）)

机构地区：[1]浙江省肿瘤医院检验科,杭州310022 [2]浙江省绍兴市人民医院临床检验中心,312000 [3]浙江省医学科学院病毒病研究所,杭州310013

出　　处：《中华临床感染病杂志》2018年第1期42-46,共5页Chinese Journal of Clinical Infectious Diseases

基　　金：浙江省中医药科技计划科研基金项目（2015ZA041）

摘　　要：目的观察穿心莲提取物对柯萨奇病毒A16型（CVA16）的体外抑制作用。方法采用CCK8法检测浓度为500.0、250.0、125.0、60.0、30.0、15.0、7.5和3.8 μg/mL的穿心莲提取物对非洲绿猴肾上皮细胞（Vero细胞）的毒性作用，用概率单位回归法计算半数抑制浓度（IC50）。以利巴韦林（RBV）为药物对照，比较穿心莲提取物对CVA16感染Vero细胞的直接灭活病毒、阻断病毒吸附和抑制病毒复制作用，采用SPSS 24.0软件对数据进行分析。结果根据细胞毒性试验选取药物溶度均分别为15.0、7.5和3.8 μg/mL的穿心莲提取物和利巴韦林进行抗CVA16实验。穿心莲提取物与利巴韦林在3个浓度下对CVA16既无直接灭活作用，也无阻断病毒吸附作用（F=1.54、1.52和0.67、1.68，P值均〉0.05），两种药物在7.5和15.0 μg/mL时均能抑制CVA16在Vero细胞的复制（t=6.87、11.76和7.71、12.84，P〈0.05）。结论体外实验表明，穿心莲提取物可通过抑制CVA16在Vero细胞内的复制，实现抗病毒作用。Objective To investigate the effect of Andrographis paniculata Nees （APN） extract on Coxsackievirus A16 （CVA16） in vitro.Methods African green monkey kidney-derived Vero cells （Vero cells） were treated with APN extract at the concentration of 500.0, 250.0, 125.0, 60.0, 30.0, 15.0, 7.5 and 3.8 μg/mL, the cytotoxicity was determined with cell counting Kit-8 and the IC50 was calculated by Probit unit regression method. Direct inactivating activity on CVA16, blocking of CVA16 adsorbing Vero cells and inhibition of CVA16 replication in Vero cells were determined and compared between Ribavirin （RBV） and APN extract with CVA16-infected Vero cells. SPSS 24.0 software was used to analyze the data.Results The selected concentrations of APN extract and RBV for experiment were 15.0, 7.5 and 3.8 μg/mL according to cytotoxicity test. Both of APN extract and RBV had neither direct inactivation on CVA16 nor blocking of CVA16 adsorbing at the concentration of 15.0, 7.5 and 3.8 μg/mL（F=1.54, 1.52 and 0.67, 1.68, all P〉0.05）. However, both drugs had the capability of inhibiting CVA16 replication in Vero cells at the concentration of 15.0 and 7.5 μg/mL（t=6.87, 11.76 and 7.71, 12.84, all P〈0.05）.Conclusion Experimental result shows that APN extract can effectively inhibit CVA16 replication in Vero cells in vitro.

关 键 词：柯萨奇病毒感染 穿心莲属 致细胞病变 病毒 抗病毒作用 

分 类 号：R512.5[医药卫生—内科学]