胰弹力蛋白酶联合血管紧张素Ⅱ制作兔腹主动脉瘤模型的效果观察  

Establishment of abdominal aortic aneurysm model induced by pancreatic elastase and angiotensin Ⅱ in rabbits

作　　者：聂毛晓[1] 张学慧[1] 闫云峰[1] 冯婷婷[1] 赵欣[1] 赵全明[1] Nie Maoxiao, Zhang Xuehui, Yan Yunfeng, Feng Tingting, Zhao Xin, Zhao Quanming(Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029 ,, Chin)

机构地区：[1]首都医科大学附属北京安贞医院心内科,100029

出　　处：《中国医药》2018年第3期448-452,共5页China Medicine

基　　金：国家自然科学基金(81370437)~~

摘　　要：目的观察胰弹力蛋白酶腔内灌注联合血管紧张素Ⅱ皮下持续泵入制作兔腹主动脉瘤模型的效果。方法选取健康新西兰雄性白兔20只,随机分为A组和B组,每组10只。制备腹主动脉瘤模型,A组经导管给予腹主动脉内灌注弹力蛋白酶,B组给予腹主动脉内灌注弹力蛋白酶联合皮下持续泵注血管紧张素Ⅱ。分别于术前及术后2、4、16周测血压,行腹主动脉彩色超声检查,并检测血清基质金属蛋白酶2(MMP-2)和MMP-9水平;16周时处死兔,取血管标本行苏木精-伊红染色、EVG弹力纤维染色、Masson染色及免疫组织化学CD_(68)巨噬细胞染色。结果术后2、4、16周时,B组血压明显高于A组[(92±9)mmHg(1 mmHg=0.133 kPa)比(73±7)mmHg、(105±9)mmHg比(74±6)mmHg、(102±10)mmHg比(73±7)mmHg](P<0.05)。术后2周时,2组成瘤率及扩张率差异无统计学意义(P>0.05);术后4、16周时,A组和B组成瘤率及瘤体直径扩张率差异有统计学意义(4周:5/7比10/10、68.0%比96.0%,16周:1/7比10/10、12.0%比104.0%)(P<0.05)。术后2周时2组MMP-2、MMP-9水平均明显高于术前,术后4、16周时仅B组MMP-2、MMP-9水平明显高于术前;术后4、16周时,B组MMP-2、MMP-9水平明显高于A组[4周:(31.4±0.7)μg/L比(22.9±0.5)μg/L、(83.9±3.2)μg/L比(58.6±2.5)μg/L;16周:(29.2±0.9)μg/L比(21.8±0.7)μg/L、(85.1±2.9)μg/L比(53.1±2.0)μg/L](P<0.05)。免疫组织化学染色结果显示,B组CD_(68)阳性细胞染色累积吸光度值明显低于A组[(5 624±986)比(8 937±1 021)],差异有统计学意义(P<0.05)。结论胰弹力蛋白酶腔内灌注联合皮下持续泵入血管紧张素Ⅱ是一种可靠的制作兔腹主动脉瘤模型的方法,可使腹主动脉瘤较长时间维持瘤体的形态。Objective To observe the effect of intra-abdominal aortic injection of pancreatic elastase combined with continuous subcutaneous injection of angiotensin Ⅱ on abdominal aortic aneurysm model in rabbits. Methods Twenty male New Zealand white rabbits were randomly divided into group A and group B, with 10 rabbits in each group. Group A was infused pancreatic elastase to make abdominal aortic aneurysm model; group B had abdominal aortic infusion of pancreatic elastase combined with subcutaneous pumping of angiotensin Ⅱ. Blood pressure and serum levels of matrix metalloproteinase（MMP）-2 and MMP-9 were measured before and 2, 4, 16 weeks after operation. The rabbits were executed 16 weeks after operation; vascular tissues were separated for hematoxylin eosin, EVG elastic fiber, Masson and immunohistochemical staining tests. Results Blood pressure in group B was significantly higher than that in group A at 2, 4, 16 weeks after operation[ （92 ±9）mmHg vs （73 ± 7 ） mmHg, （ 105 ± 9） mmHg vs （ 74 ± 6 ） mmHg, （ 102±10 ） mmHg vs （ 73 ± 7 ） mmHg ] （ P 〈 0. 05 ）. Aneurysm formation rate and diameter expansion rate had no significant differences between group A and group B at 2 weeks after operation（ P 〉 0.05 ）. At 4, 16 weeks after operation, aneurysm formation rate and diameter expansion rate in group A were significantly lower than those in group B （4 weeks ： 5/7 vs 10/10, 68.0% vs 96. 0% ; 16 weeks ： 1/7 vs 10/10, 12. 0% vs 104. 0% ）（P 〈0. 05）. Serum MMP-2 and MMP-9 levels 2 weeks after operation were significantly higher than those before operation in both groups ; serum MMP-2 and MMP-9 levels 4, 16 weeks after operation were significantly higher than those before operation in group B; serum MMP-2 and MMP-9 levels 4, 16 weeks in group B were significantly higher than those in group A [ 4 weeks ： （ 31.4 ±0. 7 ） p,g/L vs （ 22. 9 ± 0. 5 ） μg/L, （ 83.9 ± 3.2） μg/L vs （ 58. 6 ± 2. 5 ） μg/L; 16 weeks ： （ 29. 2 ± 0. 9 ） μg/L vs （ 21.8

关 键 词：腹主动脉瘤 弹力蛋白酶 血管紧张素Ⅱ 兔模型 

分 类 号：R654.3[医药卫生—外科学]