水蛭素抗痛风作用及其机制研究  

Anti-gout effect of hirudin and its mechanism

作　　者：刘喜华[1] 赵应学[2] 周元明 黄敏琪[1] 黄思诗[1] 甄汉深 熊万娜[1] 刘笑[1] 刘安韬[1] LIU Xi-hua1, ZHAO Ying-xue2, ZHOU Yuan-ming1, 3, HUANG Min-qi1, HUANG Si-shix, ZHEN Han-shen4, XIONG Wan-na1, LIU Xiao1, LIU An-tao1(1. Guangxi Medical College, Nanning 530023, China 2. Guangxi Jiangbin Hospital, Nanning 530021, China 3. Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, China 4. Guangxi University of Chinese Medical, Nanning 530001, Chin)

机构地区：[1]广西卫生职业技术学院,广西南宁530023 [2]广西壮族自治区江滨医院,广西南宁530021 [3]广西中医药大学附属瑞康医院,广西南宁530011 [4]广西中医药大学,广西南宁530001

出　　处：《中草药》2018年第6期1365-1370,共6页Chinese Traditional and Herbal Drugs

基　　金：广西自然科学基金资助项目(2015GXNSFBA139150);广西教育厅科研项目(2017KY1228);广西科学研究与技术开发重大专项计划(桂科重:1355001-5-9)

摘　　要：目的探讨水蛭素抗痛风作用及其机制。方法利用次黄嘌呤复制小鼠高尿酸血症模型,尿酸钠诱导大鼠急性痛风性炎症模型,观察水蛭素对急性高尿酸血症尿酸水平及其诱导炎症的影响;利用氧嗪酸钾诱导小鼠慢性高尿酸血症模型,观察水蛭素对慢性高尿酸血症小鼠血清尿酸、尿素氮水平及血清、肝脏黄嘌呤氧化酶活性,肾脏的病理学改变、C-反应蛋白(CRP)水平及葡萄糖转运体9(GLUT9)表达的影响。结果水蛭素可显著降低次黄嘌呤引起的高尿酸血症小鼠血清尿酸水平,显著抑制由尿酸钠引起的大鼠足跖急性肿胀;可显著降低由氧嗪酸钾引起的慢性高尿酸小鼠血清高尿酸水平,降低血清尿素氮水平,显著抑制GLUT9的表达,减轻肾脏病理学损伤。结论水蛭素具有显著的抗高尿酸血症及抗痛风作用,其机制可能与保护肾脏作用及调节GLUT9表达有关。Objective To investigate the anti-gout effect of hirudin and its mechanism. Methods Hypoxanthine was used to replicate mouse model of hyperuricemia. Sodium uric induced acute gouty inflammation in rats to observe the effect of hirudin on the level of uric acid and inflammation induced by acute hyperuricemia. The serum uric acid, serum urea nitrogen, serum xanthine oxidase activity, and liver xanthine oxidase activity were observed in chronic hyperuricemia mouse model induced by potassium oxonate. The changes of renal pathology, the level of C-reactive protein（CRP） and the expression of glucose transporter 9（GLUT9） were also be tested. Results Hirudin could significantly reduce the serum uric acid level in hyperuricemia mice induced by hypoxanthine and significantly inhibit acute toe swelling induced by sodium uric acid in rats. It could significantly reduce the level of serum hyperuricemia, decrease the level of blood urea nitrogen（BUN）, significantly inhibit the expression of GLUT9 and alleviate the pathological changes of kidney in chronic hyperuricemia mice induced by potassium oxazinate. Conclusion Hirudin has significant anti-hyperuricemia and anti-gout effects, and its mechanism may be related to the protection of kidney and the regulation of GLUT9 expression.

关 键 词：水蛭素 金边蚂蟥 高尿酸血症 痛风 葡萄糖转运体9 抗炎作用 

分 类 号：R285.5[医药卫生—中药学]