外周血Th17细胞及Treg细胞检测对系统性红斑狼疮病情的评估价值  

Value of determining Th17 and regulatory T cells in the peripheral blood in evaluating patients with systemic lupus erythematosus

作　　者：郝慧琴 张少然[2,3] 李小峰 陈俊伟[4] 罗静[4] 吕志勤[4] HAO Hui-qin, ZHANG Shao-ran, LI Xiao-feng, CHEN Jun-wei, LUO Jing, LYU Zhi-qin.(Department of Rheumatology, Shanxi University of Traditonal Chinese Medicine, Taiyuan 030012, Chin)

机构地区：[1]山西中医药大学,山西太原030619 [2]山西省医学会,山西太原030001 [3]山西省卫生和计划生育委员会 [4]山西医科大学第二医院,山西太原030001

出　　处：《中国实用内科杂志》2018年第3期242-244,共3页Chinese Journal of Practical Internal Medicine

基　　金：山西省科技攻关项目(20140313012-3)

摘　　要：目的探讨外周血Th17细胞及Treg细胞检测对系统性红斑狼疮(systemic lupus erythematosus,SLE)病情评估的价值。方法以2014年1月至2016年6月在山西医科大学第二医院风湿科就诊的100例SLE患者(非活动组患者37例,活动组患者63例)及30名健康者为研究对象。采用分选流式细胞仪检测外周血Th17细胞、Treg细胞百分数,采用流式细胞仪微球捕获芯片技术检测血清白介素-17(interleukin-17,IL-17)浓度,ELISA方法检测血清转化生长因子β(transforming growth factorβ,TGF-β)浓度。结果 (1)SLE患者组Th17细胞百分数显著高于正常对照组(P<0.05),且活动组Th17细胞显著高于非活动组(P<0.05)。(2)SLE患者组Treg细胞显著低于正常对照组(P<0.05),SLE活动组Treg细胞显著低于非活动组(P<0.05)。(3)SLE活动组Th17/Treg显著高于SLE非活动组与正常对照组(P<0.05)。(4)SLE患者组IL-17显著高于正常对照组(P<0.05),活动组IL-17显著高于非活动组(P<0.05);患者组与正常对照组、SLE活动组与非活动组TGF-β差异均无统计学意义(P>0.05)。结论 Th17细胞及Treg细胞参与SLE的发生、发展,Th17/Treg可以作为SLE疾病活动和病情评价的可靠指标。Objective To study the value of determining Th17 and regulatory T cell（Treg）in the peripheral blood in evaluating patients with systemic lupus erythematosus（SLE）. Methods This study included 100 patients with SLE （37 patients were in inactive SLE group and 63 patients were in active group） and 30 normal controls. Percentage of Th17 and Treg in the peripheral blood were detected by flow cytometry. The concentration ofinterleukin-17 was detected by cytometric bead array（CBA）. The concentration of transforming growth factor-β was detected by ELISA. Results （1）The percentage of Th17 in the peripheral blood in patients with SLE was higher than that in normal controls （P〈0.05）, and the percentage of Th17 in active SLE patients was higher than that in inactive SLE patients （P〈0.05）. （2）The percentage of Treg in patients with SLE was lower than that in normal controls（P〈0.05）, and the percentage of Treg in active SLE was lower than that in inactive SLE patients（P〈0.05）. （3）Tote percentage of Th17/Treg level in active SLE patients was higher than that in inactive SLE patients and normal controls（P〈0.05）. （4）The concentration ofinterleukin-17 in patients with SLE was higher than that in normal controls （P〈0.05）, and the concentration of interleukin-17 in active SLE patients was higher than that in inactive SLE patients and normal controls（P〈0.05）. The concentration of TGF-β had no difference between SLE patients and normal controls（P〉0.05）, and it had no difference between active SLE patients and inactive SLE patients（P〉0.05）. Conclution Th17 and Treg contribute to the pathogenesis of SLE, and Thl7/Treg level can be the reliable index of SLE condition evaluation.

关 键 词：红斑狼疮 系统性 T淋巴细胞 辅助诱导 流式细胞术 

分 类 号：R593.24[医药卫生—内科学]