香蕉不定芽^(60)Co辐射诱变研究  

Radiation-induced Mutation of Banana Adventitious Shoots by using ^(60)Co

作　　者：张建斌[1] 张建平[1] 王安邦[2] 徐碧玉[1] 金志强[1,2] 刘菊华[1] ZHANG Jianbin1,ZHANG Jianping1,WANG Anbang2,XU Biyu1, JIN Zhiqiang1,2, LIU Juhua1(1 Institute of Tropical Bioscience and Biotechnology, CATAS/Ministry of Agriculture Key Biotechnology Laboratory for Tropical Crops, Haikou, Hainan 571101; 2 Hainan Key Laboratory of Genetic Improvement of Bananas / Haikou Experimental Station, CATAS, Haikou, Hainan 57010)

机构地区：[1]中国热带农业科学院热带生物技术研究所/农业部热带作物生物技术重点开放实验室,海南海口571101 [2]海南省香蕉遗传改良重点实验室/中国热带农业科学院海口实验站,海南海口570102

出　　处：《热带农业科学》2018年第2期42-45,51,共5页Chinese Journal of Tropical Agriculture

基　　金：海南耕地改良关键技术与示范专项(No.HNGDpz201502);国家香蕉产业技术体系(No.CARS-31);海南省农村综合改革示范试点专项

摘　　要：以香蕉未成熟雄花诱导的不定芽为受体,采用钴60辐射诱变技术,研究不同辐射剂量对不定芽和植株成活率的影响,并分析突变植株的表型。结果表明:巴西香蕉未成熟雄花诱导的不定芽的适宜辐射剂量范围为3~7 KR,以5 KR为最佳,得到的有益突变率最多。本研究结果为通过辐射诱变技术培育香蕉新种质提供依据。Adventitious shoots induced from banana immature male flower were radiated with a cobalt-60 （^60Co） source to induce mutagenesis to observe the effects of different radiation doses on the survival rates of shoots and plantlets, and their mutant phenotypes were analyzed. The results indicated that the suitable radiation dose of ^60Co for banana adventitious shoots ranged from 3-7 KR, with the dose of 5 KR being the optimum, which produced the most beneficial mutations. This result will provide basis for radiation-induced mutation of banana new germplasm.

关 键 词：香蕉 ^60Co辐射诱变 有益突变 

分 类 号：S668.1[农业科学—果树学]