抗α-胞衬蛋白、抗SSA抗体、抗SSB抗体联合检测在干燥综合征的诊断价值  

The diagnosis and the clinical significance of combined detection of anti-α-Fordrin、 SSA and SSB in Sjgren’s syndrome

作　　者：张孝丰 汪一萍 

机构地区：[1]宁波鄞州人民医院检验科

出　　处：《浙江检验医学》2011年第4期17-19,共3页Zhejiang Journal of Laboratory Medicine

摘　　要：目的探讨抗SSA抗体、抗SSB抗体,抗α-胞衬蛋白(α-Fordrin)联检在干燥综合征诊断中的价值。方法收集本院190例干燥征患者及健康对照者的血清,以间接免疫荧光法检测ANA,ELISA定量检测(SS)α-胞衬蛋白抗体水平,蛋白印迹法检测SSA、SSB,定性结果阳性率以百分率表示,结果间采用χ2检验。结果 ANA、α-Fordrin-IgG抗体、SSA、SSB在原发性干燥综合征组的阳性率分别为65.7%,40%,34.3%,71.4%,40%,在继发性干燥综合征阳性率为92%,24%,8%,32%,32%,ANA增高,其他四项自身抗体也显著高于其他结缔组织病与健康体检者,差异有统计学意义;SSA、SSB、α-Fordrin三者联合检测时阳性率可达88.6%;在SSA或SSB抗体任一阴性的患者中,α-Fordrin抗体阳性率分别达50%和42.9%,但结果未见差异。结论α-胞衬蛋白与SSA、SSB两两联合检测或三种抗体联合检测互为补充,更有助于干燥综合征的诊断。Objective To investigate the diagnostic significance of combined detection of anti-α-Fordrin、 SSA and SSB in Sjgren’s syndrome(SS).Methods 190 serum samples were obtained from diseases and healthy individuals.ANA was tested by indirect immunoflurescence;the presence of anti-F5-IgA antibody was examined by enzyme-linked immunsorbent assay;SSA、SSB antibody was tested by immunoblotting technique;then analyse the results with statistical software.Results 1.For the detection of autoantibody,the postitive rate in pSS was 65.7%,40%,34.3%,71.4%,40%respectively,the postitive rate in sSS was92%,24%,8%,32%,32% respectireey;which were significantly higher than those in non-CTD persons and normal controls except for ANA.2.The combined determination of anti-α-Fordrin、 SSA and SSB antibody could improve the determination percentage to 88.6%.3.Anti-α-Fordrin antibodies were present in 50% and 42.9%of pSS persons with negative anti-SSA and anti-SSB respectively.Conclusion The combined determination of anti-α-Fordrin、 SSA and SSB antibody could improve the determination percentage in Sjogren’s syndrome.

关 键 词：α-胞衬蛋白SSA SSB 联合检测 干燥综合征 

分 类 号：R446.62[医药卫生—诊断学]