交泰丸对慢性温和不可预知性应激抑郁模型大鼠炎性细胞因子的影响  

Effects of Jiaotai Pill on inflammatory cytokines in depression model mice with chronic unpredictable mild stress

作　　者：梁如[1] 殷佳[1] 潘晔[1] 周好波 高树明[1] 于春泉[1] LIANG Ru, YIN Jia, PAN Ye, ZHOU Hao-bo, GAO Shu-ming, YU Chun-quan(Tianjin University of Traditional Chinese Medicine, Tianjin 300193, Chin)

机构地区：[1]天津中医药大学,天津300193

出　　处：《中草药》2018年第5期1100-1105,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金面上项目(81273896)

摘　　要：目的探讨交泰丸对慢性温和不可预知性应激(CUMS)抑郁模型大鼠致炎、抗炎细胞因子的影响。方法大鼠采用CUMS方法制备抑郁模型,比较体质量变化,进行糖水消耗实验和开场实验;并采用酶联免疫吸附测定法(ELISA)测定大鼠血清及海马组织中炎性细胞因子白细胞介素(IL)-1β、IL-6、肿瘤坏死因子(TNF)-α、IL-4、IL-10的水平,尼氏染色法观察大鼠海马神经元形态。结果与对照组比较,模型组大鼠糖水消耗量、穿越横格数和直立次数均明显减少;血清及海马内IL-1β、IL-6、TNF-α、IL-4明显升高,IL-10明显降低;海马神经元分层不清,排列紊乱,尼氏体浅染或消失。与模型组比较,交泰丸给药组大鼠糖水消耗量、穿越横格数和直立次数均明显增加;血清及海马内IL-1β、IL-6、TNF-α明显降低,IL-4、IL-10明显升高;海马神经元分层较明显,尼氏体浅染。结论交泰丸可明显改善大鼠抑郁样行为及海马神经元损伤,其机制可能与下调血清及海马致炎细胞因子IL-1β、IL-6、TNF-α和上调抗炎细胞因子IL-4、IL-10表达有关。Objective To study the mechanism of Jiaotai Pill on inflammation and anti-inflammatory cytokines in mice with chronic unpredictable mild stress（CUMS） depression. Methods Mice were treated with CUMS depression model, then the weight comparison, sugar test, opening experiment was conducted; The levels of IL-1β, IL-6 and TNF-α, IL-4, and IL-10 in the serum and hippocampus of rats were measured by enzyme-linked immunosorbent assay（ELISA）, and rat hippocampal neuron morphology was observed by Nissl’s staining. Results Compared with the blank group, the consumption of sugar, the number of crosses and the number of vertical bars in the model group were significantly decreased; The levels of IL-1β, IL-6, TNF-α, and IL-4 in serum and hippocampus were significantly higher than those in the blank group, and IL-10 significantly reduced; Hippocampal neurons stratified unclear, arranged disorder, Nissie shallow staining or disappear. Compared with the model group, the consumption of sugar, the number of crosses and the number of vertical bars in Jiaotai Pill administration group were significantly increased; The levels of IL-1β, IL-6, and TNF-α in serum and hippocampus were significantly decreased, and the levels of IL-4 and IL-10 was significantly increased; Hippocampal neuronal stratification was more obvious, Nissl shallow staining. Conclusion Jiaotai Pill can significantly improve the behavior of rat depression and hippocampal neuronal injury whose mechanism may be related to down-regulation of serum and hippocampus proinflammatory cytokines IL-1β, IL-6, TNF-α, up-regulation of anti-inflammatory cytokines IL-4 and IL-10.

关 键 词：交泰丸 抑郁症 慢性温和不可预知性应激抑郁模型 致炎细胞因子 抗炎细胞因子 

分 类 号：R285.5[医药卫生—中药学]