基于声光可调滤波器双滤波技术的超光谱显微成像系统及其图像分析  

Hyperspectral Microimaging System Based on Double-Filtering Technology of Acousto-Optic Tunable Filter and Its Image Analysis

作　　者：石磊 张春光 王号 原江伟 Shi Lei1,2, Zhang Chunguang1,2, Wang Hao1,2, Yuan Jiangwei1,2(1.Fujian Provincial Key Laboratory for Photonics Technology, Fuzhou, Fujian 350007, China 2 Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou, Fujian 350007, Chin)

机构地区：[1]福建省光子技术重点实验室,福建福州350007 [2]福建师范大学光电与信息工程学院医学光电科学与技术教育部重点实验室,福建福州350007

出　　处：《激光与光电子学进展》2018年第3期423-428,共6页Laser & Optoelectronics Progress

基　　金：福建省自然科学基金(2017J01745);教育部"长江学者和创新团队发展计划"创新团队项目滚动支持计划(IRT_15R10)

摘　　要：光谱分辨率是声光可调滤波器(AOTF)的关键。基于声光滤波器的工作原理,通过前后串联两个滤波器,设计了基于双滤波结构的超光谱成像系统。通过对单一滤波和双滤波结构特性的理论计算和实验测量结果的分析比较,可以发现在中心波长相等的情况下,双滤波结构的光谱宽度比单一滤波结构小,说明了双滤波技术在改善光谱宽度方面的优越性。另外,利用双滤波结构,结合倒置光学显微镜,设计了基于双滤波技术的显微成像系统。基于大量的胃癌组织超光谱显微图像,选取相关系数较小的三个光谱图像,使用RGB假彩色图像融合技术进行处理。经仿真实验发现,超光谱图像融合技术能够有效地改善图像的质量。Spectral resolution is the key of acousto-optie tunable filter （AOTF）. Based on the working principle of acousto-optic filter, a hyperspectral imaging system based on double-filter structure is designed by connecting two filters in series. By comparing the theoretical calculation and experimental measurement results of single filter and double filter, it can be found that the spectral width of double filter structure is smaller than that of single filter when the center wavelength is equal, which shows that the double filter technology can improve the spectral width. In addition, the microscopic imaging system based on double-filter technology is designed by using dual-filter structure combined with inverted light microscope. Based on a large number of microscopic images of gastric cancer tissue hyperspectral images, three spectral images with a small correlation coefficient are selected and processed using RGB false color image fusion technology. The simulation results show that the hyperspectral image fusion technology can effectively improve the image quality.

关 键 词：光学器件 声光可调滤波器 双滤波技术 数字图像处理 

分 类 号：O439[机械工程—光学工程]