锦鹤养心方总黄酮纯化工艺优选及对小鼠常压耐缺氧影响  

Optimization of Purification Process for Total Flavonoids from Jinhe Yangxin Decoction and Study the Effect on the Ability of Anoxia Endurance

作　　者：程艳刚 谭金燕 李国艳 刘艳[1] 李慧峰 孔祥鹏 郭瑞 杨炳友[1] 裴妙荣[1,2] CHENG Yangang1, TAN Jinyan1, LI Guoyan2, LIU YanI, LI Huifeng2, KONG Xiangpeng2 , GUO Rui2, YANG Bingyou1, PEI Miaorong1,2(1.Key Laboratory of Chinese Materia Medica Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang, China; 2.Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi, China)

机构地区：[1]黑龙江中医药大学,北药基础与应用研究教育部重点实验室,黑龙江哈尔滨150040 [2]山西中医药大学,山西晋中030619

出　　处：《辽宁中医药大学学报》2018年第3期34-39,共6页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81373546;30472125;81273660)

摘　　要：目的：优化大孔树脂吸附法富集锦鹤养心方总黄酮的工艺条件,并研究其对小鼠常压耐缺氧的影响。方法：以芦丁为对照品,硝酸铝显色法检测总黄酮的含量,通过静态吸附/解吸与动态吸附/解吸相结合的方法筛选大孔树脂型号,采用单因素实验考察上样量、上样流速、洗脱流速等对总黄酮纯化工艺的影响;采用常压耐缺氧实验,观察锦鹤养心方总黄酮对小鼠耐缺氧能力的影响。结果：AB-8型大孔树脂对锦鹤养心方总黄酮的纯化效果最好,最佳纯化工艺为上样液质量浓度0.5 g·m L-1,上样流速为2 BV·h-1,上样量为2 BV,吸附4 h,加水1.5 BV洗脱水溶性杂质,加50%乙醇3 BV洗脱,洗脱流速为2 BV·h-1,在此条件下,提取物中总黄酮质量分数由25.01%提高到85.54%;与空白对照组相比,锦鹤养心方总黄酮能明显增加小鼠常压耐缺氧存活时间。结论：AB-8型大孔树脂对锦鹤养心方总黄酮具有良好的纯化效果,优选工艺合理、稳定可行,且锦鹤养心方总黄酮能显著提高小鼠的耐缺氧能力。Objective：To optimize enrichment and purification technology of total flavonoids from Jinhe Yangxin Decoction and study the effect of it on the ability of anoxia endurance in mice. Methods：With rutin as reference substance,the content of total flavonoids was determined by aluminium nitrate chromogenic method,optimum macroporous resin by static adsorption/desorption and dynamic adsorption/desorption,and single factor tests were adopted to investigate effects of sample amount,sample flow rate,elution rate and other factors on purification technology;And observed the effect of total flavonoids from Jinhe Yangxin Decoction on the ability of anoxia endurance in mice by using the anoxia endurance on normal pressure test. Results：Compared with other resins,AB-8 macroporous resin showed the best separation efficiency,and the optimum purification technology was as follows：the concentration of sample solution of 0.5 g·m L-1,sampling velocity of 2 BV·h-1,sample volume of 2 BV,adsorption time of 4 h,eluted by 1.5 BV deionized water and 3 BV 50% ethanol at the speed of 2 BV·h-1,eluate was collected. Under these process conditions,the content of total flavonoids increased from 25.01% to 85.44%;And compared with the blank group,total flavonoids from Jinhe Yangxin Decoction could obviously prolong the living time of oxygen deficit-tolerance. Conclusion：AB-8 macroporous resin had a good purification effect of total flavonoids from Jinhe Yangxin Decoction,and it could obviously improve the ability of anoxia endurance.

关 键 词：大孔树脂 黄酮 芦丁 耐缺氧 

分 类 号：R284[医药卫生—中药学]