日本血吸虫感染C57BL/6小鼠脾脏调节性B细胞的检测  

Detection of regulatory B cells in the spleen of Schistosoma japonicum infected C57BL/6 mouse

作　　者：曲佳乐 冯源发 金晨曦 齐艳伟 邱怀娜 杨权 黄俊 谢红艳 QU Jia-le, FENG Yuan-fa, JIN Chen-xi, QI Yan-wei, QIU Huai-na,YANG Quan, HUANG Jun, XIE Hong-yan(School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong 511436, Chin)

机构地区：[1]广州医科大学基础学院,广东广州511436

出　　处：《热带医学杂志》2018年第3期289-293,共5页Journal of Tropical Medicine

基　　金：国家自然科学基金(81771696);广东省教育厅重大项目(2016KZDXM033)

摘　　要：目的探讨日本血吸虫感染C57BL/6小鼠脾脏不同亚群调节性B细胞的含量。方法使用日本血吸虫尾蚴经腹部感染C57BL/6小鼠,5周后分离感染组及正常对照组小鼠脾细胞,制备单个细胞悬液。使用细胞表面染色的方法,通过流式细胞术检测小鼠CD19^+B细胞含量及表达免疫调节相关分子CD5、CD11b以及PD-1的B细胞亚群的含量。结果 C57BL/6小鼠感染日本血吸虫后,脾脏B细胞的百分比含量及数目明显增多,差异有统计学意义(P<0.01);CD5^+和CD11b^+B细胞的含量明显增加,差异有统计学意义(P<0.05);而PD-1^+B细胞的含量变化不明显,差异无统计学意义(P>0.05)。结论日本血吸虫感染能在C57BL/6小鼠体内诱导CD5^+和CD11b^+调节性B细胞产生。Objective To explore the content of different subset of regulatory B cells in the spleen of Schistosoma japonicum infected C57BL/6 mouse. Methods C57BL/6 mice were percutaneously infected with Schistosoma japonicum （S. japonicum） cercariae. Five weeks later, splenocytes were isolated, and single cell solutions were made. The content of CD19＋ B cells, and the expression of Immunomodulatory related molecule： CDS, CDllb, and PD- 1 on B cells were investigated by the means of cell surface staining, and detected by Fluorescence activated cell sorting （FACs）. Results After S. japonicum infection, the percentages and numbers of B cells in the spleen were increased significantly （P〈0.01）. The percentages of CD5＋and CD11b＋ B cells were also increased （P〈0.05）. However, the changes of PD-1＋ B ceils was not so clear （P〉0.05）. Conclusion S. japonicum infection could up regulate CD5＋ and CDllb＋ regulatory B cells in the spleen of C57BL/6 mice.

关 键 词：日本血吸虫 B细胞 调节性B细胞 免疫调节分子 

分 类 号：R383.24[医药卫生—医学寄生虫学]