慢性间歇性缺氧对大鼠肝脏糖代谢的影响及机制  

Effect of chronic intermittent hypoxia on glycometabolism in rat' liver and the mechanism thereof

作　　者：余维 覃泽平 陈华娇 张峰[4] 李兵 YU Wei1, Q.IN Ze-ping2, CHEN Hua-jiao3, ZHANG Feng4, LI Bing(IDepartment of ENT, Second Affiliated Hospital of Army Medical University, Chongqing 400037, China 2Department of ENT Seventh People＇s Hospital of Chongqing, Chongqing 400054, China 3Department of ENT, Fuling Central Hospital, Chongqing 408003, China 4Department of ENT, Children＇s Hospital Affiliated to Chongqing Medical University, Chongqing 401122, China SDepartment of ENT, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, Chin)

机构地区：[1]陆军军医大学第二附属医院耳鼻喉科,重庆400037 [2]重庆市第七人民医院耳鼻喉科,重庆400054 [3]涪陵中心医院耳鼻喉科,重庆408003 [4]重庆医科大学附属儿童医院耳鼻喉科,重庆401122 [5]重庆医科大学第一附属医院耳鼻喉科,重庆400016

出　　处：《解放军医学杂志》2018年第3期206-210,共5页Medical Journal of Chinese People's Liberation Army

基　　金：重庆市卫生局重点科研项目(2012-1-059)

摘　　要：目的探讨慢性间歇性缺氧对大鼠糖代谢的影响及机制。方法 24只成年雄性SD大鼠随机均分为对照(UC)组、慢性间歇性缺氧(CIH)组及复氧(RH)组,建模完成后行动脉血气分析。采用过氧化物酶法检测各组大鼠血清中空腹血糖(FBG)、胰岛素(FINS)水平,ELISA法检测游离脂肪酸(FFA)、瘦素浓度,并分别采用q RT-PCR及Western blotting检测肝脏中葡萄糖转运蛋白2(GLUT-2)、胰岛素受体底物2(IRS-2)、瘦素mRNA及蛋白的表达水平。结果 CIH组大鼠血清FBG、FINS、FFA及瘦素浓度明显高于UC组(P<0.05),而RH组则明显高于CIH组(P<0.05)。Western blotting及q RT-PCR检测结果显示,CIH组大鼠肝脏GLUT-2、IRS-2蛋白及mRNA表达明显低于RH组(P<0.05),而RH组明显低于UC组(P<0.05);CIH组大鼠肝脏瘦素蛋白及mRNA的表达明显高于RH组(P<0.05),而RH组则明显高于UC组(P<0.05)。结论慢性间歇性缺氧导致的胰岛素抵抗可能与大鼠血清FFA、瘦素升高以及肝脏GLUT-2、IRS-2表达降低,瘦素表达升高相关。Objective To investigate the effects of chronic intermittent hypoxia on the adipose factor and the expressions of insulin receptor substrate 2（IRS-2）, glucose transporter 2（GLUT-2） and leptin in rat liver. Methods Twenty-four mature SD rats were randomly divided into 3 groups： control group（UC）, chronic intermittent hypoxia group（CIH） and reoxygenation group（RH）. The arterial blood gas analysis was performed after the establishment of rat model. The serum fasting blood glucose（FBG） and fasting insulin（FINS） in each group were detected by peroxidase method; the concentrations of free fatty acids（FFA） and leptin were detected by ELISA. The expressions of mRNA and protein of GLUT-2, IRS-2 and leptin were detected by q RT-PCR and Western blotting. Results The serous concentrations of FBG, FINS, FFA and leptin were significantly higher in CIH group than in UC group（P0.05）, and were dramatically higher in RH group than in both CIH group（P=0.003） and UC group（P=0.000）. Western blotting and q RT-PCR detection showed that the protein and mRNA expressions of GLUT-2 and IRS-2 were significantly lower in CIH group than in RH group of rat liver（P0.05）, while were markedly lower in RH group than in UC group（P0.05）; the expressions of leptin protein and mRNA were significantly higher in CIH group than in RH group（P0.05）, while were obviously higher in RH group than in UC group of rat liver（P0.05）. Conclusion Insulin resistance induced by chronic intermittent hypoxia may be associated with the elevation of serum FFA and leptin, and be related to the decreased expression of GLUT-2 and IRS-2 and increased expression of leptin in liver.

关 键 词：慢性间歇性缺氧 胰岛素抵抗 葡萄糖转运蛋白2 胰岛素受体底物2 瘦素 游离脂肪酸 

分 类 号：R766[医药卫生—耳鼻咽喉科]