Hsa_circ_PVT1在肝细胞癌中的表达及意义  

Expression of hsa_circ_PVT1 in human hepatocellular carcinoma and its clinical significance

作　　者：朱元鑫 付航玮[1] 林夏 蓝翔[1] 马钰 李光耀[1] 董睿 王宇洲 陈平[1] ZHU Yuan-xin, FU Hang-wei, LIN Xia, LAN Xiang, MA Yu, LI Guang-yao, DONG Rui, WANG Yu-zhou, CHEN Ping(Department of Hepatobiliary Surgery Institute of Surgery Research, Daping Hospital, Army Medical Universityj Chongqing 400042, Chin)

机构地区：[1]陆军军医大学大坪医院野战外科研究所肝胆外科,重庆400042

出　　处：《解放军医学杂志》2018年第3期211-216,共6页Medical Journal of Chinese People's Liberation Army

摘　　要：目的探讨人肝细胞癌(HCC)中circ-PV T1的表达及其对肝癌细胞增殖的影响,并探讨其临床意义。方法利用RT-qPCR技术检测46例人肝癌组织/癌旁组织中circ-PVT1的表达水平,并且分析病理指标与其表达水平的关系;体外培养人正常肝细胞系(L02)、人肝癌细胞系(HepG2、SMMC-7721、MHCC-97H、MHCC-97L、HCC-LM3),利用RT-qPCR技术检测circ-PVT1的表达水平。利用脂质体转染技术体外转染si-circ PVT1,敲低circ-PVT1在HepG2、SMMC-7721细胞系中的表达量,同时设置阴性对照组,通过CCK-8实验、EdU实验检测干扰circ-PVT1表达对肝癌细胞增殖的影响;采用流式细胞术观察干扰circ-PVT1表达对肝癌细胞周期的影响。结果 circ-PVT1在人肝癌组织中表达量明显高于癌旁组织(P<0.001),且其表达水平与肿瘤大小、TNM分期及分化程度相关;同样,肝癌细胞系HepG2、SMMC-7721、MHCC-97H、MHCC-97L、HCC-LM3中circ-PV T1表达水平也明显高于正常肝细胞系L02(P<0.05);与阴性对照组比较,circ-PVT1干扰组HepG2及SMMC7721的增殖能力明显降低。结论在人HCC的诊断中,circ-PVT1可能作为一种潜在的生物标志物予以应用,并且可能成为一种新型的增殖因子。Objective To determine the expression and clinical significance of circ-PV T1 in human hepatocellular carcinoma（HCC） and its effect on HCC cell proliferation. Methods The expressions of circ-PVT1 in hepatocellular carcinoma and the matched tumor-adjacent tissues were detected by RT-q PCR and the relationship between pathological indexes and the expression level was analyzed in 46 patients. The expressions of circ-PVT1 in human normal liver cell line（L02） and hepatocellular carcinoma cell lines（HepG2, SMMC-7721, MHCC-97 H, MHCC-97 L, HCC-LM3） were detected by RT-q PCR and were compared thereafter. With knocking down the expression of circ-PVT1, si-circ PVT1 was transfected into HepG2 and SMMC-7721 cells by using lipofectamine technique in vitro, with the si-NC being taken as negative control. After interfering the expression of circ-PVT1, the effect on the proliferation of hepatocellular carcinoma cells was detected by CCK-8 and EDU experiments and flow cytometry was conducted to obser ve the effect of circ-PVT1 on cell cycle. Results The expression level of circ-PVT1 was significantly higher in HCC tissues than in adjacent tissues（P0.01）, and its high expression level was significantly correlated with tumor size, TNM stage and differentiation degree. Similarly, in human hepatocellular carcinoma cell lines（HepG2, SMMC-7721, MHCC-97 H, MHCC-97 L, HCC-LM3）, the expression level of circ-PVT1 was also higher than that in human normal liver cell line L02（P0.05）. Compared with the negative control group, silencing of circ-PVT1 resulted in remarkable reduction in cell proliferation of HepG2 and SMMC-7721. Conclusion circ-PVT1 may act as a potential biomarker for HCC diagnosis and may become a novel proliferation factor.

关 键 词：肝细胞癌 环状RNA PVT1 细胞增殖 细胞周期 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学]