HPLC波长切换法同时测定麝香心脑乐片中7种成分  

Simultaneous determination of seven constituents in Shexiang Xinnaole tablets by HPLC wavelength switching method

作　　者：沈静琳 丁莹 SHEN Jing-lin1 , DING Ying2(1. Department of Pharmacy, Jiangxi Medical College, Shangrao 334000, China; 2. Medical Institute of Chemical Industry, Guangdong Pharmaceutical University, Zhongshan 528458, China)

机构地区：[1]江西医学高等专科学校药学系,上饶334000 [2]广东药科大学医药化工学院,中山528458

出　　处：《药物分析杂志》2018年第3期520-526,共7页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立HPLC波长切换法同时测定麝香心脑乐片中羟基红花黄色素A、3′-羟基葛根素、葛根素、3′-甲氧基葛根素、丹参素、丹酚酸B和丹参酮ⅡA的含量,为麝香心脑乐片质量的全面评价提供科学依据。方法:该药物70%甲醇提取液采用Hypersil BDS C_(18)色谱柱(250 mm×4.6 mm,5 um),以乙腈(A)-0.4%磷酸溶液(B)为流动相,梯度洗脱。结果:羟基红花黄色素A、3′-羟基葛根素、葛根素、3′-甲氧基葛根素、丹参素、丹酚酸B和丹参酮ⅡA的线性范围分别在1.98~39.60、2.86~57.20、6.14~122.80、2.17~43.40、2.05~41.00、10.68~213.60、2.12~42.40μg·mL^(-1)内,与色谱峰峰面积呈良好的线性关系;平均加样回收率均在97.0%~99.8%,RSD均≤1.8%(n=6)。结论:本文建立的样品处理简便,测定结果准确,重复性好,可用于麝香心脑乐片质量评价。Objective： To develop an HPLC wavelength switching method for determining the contents of hydroxysafflor yellow A, 3＇-hydroxy puerarin, puerarin, 3＇-methoxy puerarin, danshensu, salvianolic acid B and tanshinone ⅡA in Shexiang Xinnaole tablets simultaneously.It provides a scientific evaluation for comprehensive quality control of Shexiang Xinnaole tablets.Methods： The analysis of 70% methanol extract of the drug was performed on Hypersil BDS C18 （250 mm×4.6 mm, 5 um） chromatographic column;the mobile phase was acetonitrile（A） -0.4% phosphoric acid solution （B） with gradient elution.Results： The calibration curves of hydroxysafflor yellow A, 3＇-hydroxy puerarin, puerarin, 3＇-methoxy puerarin, danshensu, salvianolic acid B and tanshinoneⅡA showed good linearity in the range of 1.98-39.60, 2.86-57.20, 6.14-122.80, 2.17-43.40, 2.05-41.00, 10.68-213.60, 2.12-42.40 μg·mL-1, respectively.The average recoveries were 97.0%-99.8%;and RSDs were not more than 1.8% （n=6）.Conclusion： An HPLC wavelength switching method has been successfully established for the simultaneous determination of 7 constituents in Shexiang Xinnaole tablets.The sample processing method is simple and the results are accurate and repeatable.It can be used for quality control of Shexiang Xinnaole tablets.

关 键 词：麝香心脑乐片 羟基红花黄色素 羟基葛根素 葛根素 甲氧基葛根素 丹参素 丹酚酸 丹参酮 液相色谱法 

分 类 号：R917[医药卫生—药物分析学]