大黄、枳实、厚朴不同炮制品对小承气汤中药效组分的影响  

Effect of Rhei Radix et Rhizoma, Aurantii Fructus Immaturus, and Magnoliae Officinalis Cortex different processed products on active components in Xiaochengqi Decoction

作　　者：郭亚芳[1] 王佳琳[1] 张贵君[1,2] 彭慧 刘亮 刘傲雪 王月 GUO Ya-fang1, WANG Jia-linl, ZHANG Gui-jun1,2 PENG Hui2, LIU Liang2, L1U Ao-xue1, WANG Yue1(1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China 2. Zibo Wanjie Institute of Chinese Medicine, Zibo 255213, Chin)

机构地区：[1]北京中医药大学中药学院,北京100102 [2]淄博万杰中医药研究所,山东淄博255213

出　　处：《现代药物与临床》2018年第3期456-463,共8页Drugs & Clinic

摘　　要：目的研究大黄、枳实、厚朴饮片变化对小承气汤药效组分的影响,以期为临床的合理应用和饮片的质量标准提供参考。方法采用HPLC法分别测定各类成分。大黄游离蒽醌类(芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚):Syncronis C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相:甲醇–0.1%磷酸;梯度洗脱;体积流量0.8 m L/min;柱温30℃;进样量10μL;检测波长254 nm。大黄结合蒽醌类(番泻苷B、番泻苷A):Syncronis C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈–0.05%磷酸;梯度洗脱;柱温30℃;进样量10μL;检测波长340 nm。枳实黄酮苷类(芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷):Syncronis C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈–水;梯度洗脱;体积流量0.7 m L/min;柱温40℃;进样量10μL;检测波长283 nm。厚朴木脂素类成分(和厚朴酚、厚朴酚):Syncronis C_(18)色谱柱(250 mm×4.6mm,5μm);流动相:乙腈–0.05%磷酸;梯度洗脱;体积流量0.7 m L/min;柱温30℃;进样量10μL;检测波长294 nm。结果小承气汤配伍剂量(大黄12 g–炒枳实9 g–姜厚朴6 g)不变,大黄、枳实、厚朴饮片改变时,小承气汤药效组分总量变化规律为:大黄–枳实–姜厚朴>酒大黄–炒枳实–姜厚朴>熟大黄–炒枳实–姜厚朴>大黄炭–炒枳实–姜厚朴≈小承气汤>大黄–炒枳实–厚朴,变化率分别为酒大黄组(6.561%)、熟大黄组(4.222%)、大黄炭组(0.118%)、枳实组(30.186%)、厚朴组(-11.218%)。除大黄炭组外,其余组的药效组分总量皆明显变化,其中枳实组变化最明显。结论同一味药材的不同炮制品在小承气汤处方中药效组分不同,对其他药味的影响亦不同,在小承气汤处方配伍中不可随意替代。Objective To study the effect of Rhei Radix et Rhizoma,Aurantii Fructus Immaturus,and Magnoliae Officinalis Cortex on active components in Xiaochengqi Decoction,so as to provide reference for clinical rational application and quality standards of pieces.Method The contents of various components were determined by HPLC method.The conditions of free anthraquinones（aloeemodin,rhein,emodin,chrysophanol,and physcion）were as following：the separation was performed on Syncronis C（18） column（250 mm×4.6 mm,5μm）.The mobile phase consisted of methanol-0.1%phosphoric acid with gradient elution.The detection wavelengths were set at 254 nm.The flow rate was 0.8 m L/min,temperature of column was set at 30℃,and volume of injection was10μL.The conditions of conjugated anthraquinones（sennoside A and sennoside B）were as following：the separation was performed on Syncronis C（18） column（250 mm×4.6 mm,5μm）.The mobile phase consisted of acetonitrile-0.05%phosphoric acid with gradient elution.The detection wavelengths were set at 340 nm.The temperature of column was set at 30℃,and volume of injection was 10μL.The conditions of flavonoid glycosides（rutanin,naringin,narigin,hesperidin,and neohesperidin）were as following：the separation was performed on Syncronis C（18） column（250 mm×4.6 mm,5μm）.The mobile phase consisted of acetonitrile-water with gradient elution.The detection wavelengths were set at 283 nm.The flow rate was 0.7 m L/min,temperature of column was set at 40℃,and volume of injection was 10μL.The conditions of lignans（honokiol and magnol）were as following：the separation was performed on Syncronis C（18） column（250 mm×4.6 mm,5μm）.The mobile phase consisted of acetonitrile-0.05%phosphoric acid with gradient elution.The detection wavelengths were set at 294 nm.The flow rate was 0.7 m L/min,temperature of column was set at 30℃,and volume of injection was 10μL.Results Compared with Xiaochengqi Decoction（Rhei Radix et Rhizoma 12 g-roasted Aurantii Fructus Imm

关 键 词：小承气汤 药效组分 大黄 枳实 厚朴 配伍 

分 类 号：R283.1[医药卫生—中药学]