玉米ZmNAC6基因克隆及盐碱逆境胁迫下的表达分析  

Expression Analysis of Maize ZmNAC6 Gene under Saline-alkali Stress

作　　者：何欢[1] 高嵩[1] 吕庆雪[1] 牛铭晨[1] 孙蕾[1] 李毅丹[1] 张建新[1] 王敏[1] 宋广树[1] 刘文国[1] He Huan, Gao Song, Lv Qingxue, Niu Mingchen ,Sun Lei ,Li Yidan, Zhang Jianxin ,Wang Min, Song Guangshu ,Liu Wenguo(Jilin Academy of Agricultural Sciences, Changchun, 13012)

机构地区：[1]吉林省农业科学院,长春130124

出　　处：《分子植物育种》2018年第5期1377-1381,共5页Molecular Plant Breeding

基　　金：吉林省重点科技攻关项目(20170204007NY)资助

摘　　要：在NCBI数据库中,利用水稻中耐盐碱基因OsNAC6的序列进行BLAST,获得该基因在玉米中的同源基因ZmNAC6,并对该基因进行进化树分析,同时根据该基因的CDS区设计特异引物,并对玉米杂交品种吉单96和其双亲自交系进行扩增筛选,进一步分析基因ZmNAC6在3个浓度水平的NaHCO_3(0、50 mmol/L,100 mmol/L)胁迫下的表达差异。结果表明:ZmNAC6基因在吉单96及其父本存在,实现了该基因的有效聚合,并且在盐碱胁迫下该基因在吉单96的表达量都随着盐碱浓度的升高而升高,初步判断该基因可能在吉单96的耐盐碱过程中起到正向调控作用。In the NCBI database, through BLAST for sequence of salt-tolerant gene OsNA C6 in rice, the homologous gene ZmNA C6 of maize was obtained. And the phylogenetic tree of ZmNA C6 was constructed and analysed, at the same time, the specific primer was designed according to the CDS of ZmNA C6 gene, and the hybrid maize variety Jidan 96 and its parental inbred lines were amplified and screened, as well as the expression difference of the ZmNA C6 gene in 3 different levels of NaHCO3 （0 inmol/L, 50 retool/L, 100 mmol/L） was further analyzed. The results showed that the ZmNA C6 gene was contained in Jidan 96 and male parent, so the ZmNA C6 gene was polymerized in the breeding process of Jidan 96, and the expression level increased as the NaHCO3 concentration raised, so we concluded that ZmNAC6 in Jidan 96 could play a positive role under saline-alkali stress.

关 键 词：玉米 ZmNAC6基因 电子克隆 盐碱胁迫 

分 类 号：S513[农业科学—作物学]