NaCl胁迫对转CHB101基因玉米幼苗生长的影响  

Influence of NaCl Stress on Growth of Transgenic Maize Seedlings with CHB101 Gene

作　　者：马静文[1] 王丽 李夏[1] 丁一[1] 徐长营[1] 孔祥梅[1] 祁新[3] MaJingwen1, WangLi2 ,LiXia1, DingYi1, XuChangying1, KongXiangmei1, QiXin3(1 Changchun Academy of Agricultural Science, Changchun, 130062; 2 Changchun Chaoyang District of Center for Popularization Agricultural Tech- nique, Changchun, 130000; 3 College of Agronomy, Jilin Agricultural University, Changchun, 13011)

机构地区：[1]长春市农业科学院,长春130062 [2]长春市朝阳区农业技术推广总站,长春130000 [3]吉林农业大学农学院,长春130118

出　　处：《分子植物育种》2018年第5期1524-1529,共6页Molecular Plant Breeding

基　　金：吉林省发改委玉米杂种优势表观遗传检测技术研究(2016C066)资助

摘　　要：以转CHB101基因及非转基因玉米自交系为试验材料,采用Hoagland营养液水培方法,研究在不同浓度的NaCl胁迫下,转CHB101基因玉米及非转基因玉米幼苗生长状况及生理指标差异。结果表明:转基因玉米自交系幼苗的生长状况优于对照。随着NaCl胁迫浓度的增加,转基因玉米自交系幼苗的根长、地上及地下部分含水量、根系脱氢酶活力、叶绿素含量都高于非转基因系幼苗,并且变化幅度较小;高盐胁迫下,转基因玉米幼苗的丙二醛含量变化幅度小于非转基因,脯氨酸含量、可溶性糖含量高于非转基因。以上结果说明导入的CHB101基因增强了玉米的耐盐性。In this research, we took two hybrid cultivars transgenic and non-transgenic maize seeding （CHB101） as the experimental materials to research the growth states and the difference in the physiology indexes under various concentrations of NaCl stress by using the method of Hoagland solution culture. The experimental results indicated that the growth performance of transgentic maize inbred lines was superior compared to the non-transgenic inbred lines. As the concentrations of NaCl stress increased, the indexes including root length, water content of above-ground part and underground part, activity of dehydrogenase and chlorophyll content in transgenic maize inbred lines were higher than those in non-transgenic inbred lines and the changing ranges of transgenic maize seedlings were relatively narrower, the MDA content of transgenic maize seedlings were lower than that of non-transgenic maize seeding under the high-salt stress, but the proline content and soluble sugar content were higher than those of non-transgenic maize seeding. Therefore, the target genes of CHBIO1 enhanced the salt tolerance oftransgenic maize.

关 键 词：玉米 盐胁迫 CHB101 

分 类 号：S513[农业科学—作物学]