杨梅醇与5-对氟苄氧基杨梅醇的制备及其对人肝癌HepG_2细胞活性的影响  

Preparation of Myricanol and Myricanol 5-fluorobenzyloxy Ether and its Effect on the Activity of HepG_2 Cells

作　　者：童晔玲[1] 戴关海[1] 任泽明[1] 陈璇[1] 聂晓静[1] 杨锋[1] TONG Yeling, DAI Guanhai, REN Zeming, CHEN Xuan, NIE Xiaojing, YANG Feng(Zhejiang Institute of Traditional Chinese Medicine, Hangzhou 310007, Chin)

机构地区：[1]浙江省中医药研究院,杭州310007

出　　处：《中国现代应用药学》2018年第3期375-378,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省科技计划项目(2015F10015;2013F50020;2017C33169)

摘　　要：目的体外观察杨梅醇与5-对氟苄氧基杨梅醇(myricanol 5-fluorobenzyloxy ether,5FEM)对人肝癌细胞HepG_2活性的影响。方法制备杨梅醇与5FEM,杨梅醇(25,12.5,6.25μg·m L^(-1))、5FEM(25,12.5,6.25μg·m L^(-1))作用于HepG_2细胞,实时细胞分析系统记录96 h细胞指数,观察杨梅醇与5FEM对HepG_2细胞增殖和迁移的影响。结果杨梅醇与5FEM对HepG_2细胞的增殖和迁移均有较好的抑制作用,且呈现较好的量效关系,相等剂量下,5FEM的作用优于杨梅醇。结论杨梅醇与5FEM能有效抑制HepG_2细胞的增殖和迁移。OBJECTIVE To observe the activity of myricanol and myricanol 5-fluorobenzyloxy ether（5FEM） on liver cancer of cell HepG_2 in vitro. METHODS Myricanol（25, 12.5 and 6.25 μg·m L^（-1）） and 5FEM（25, 12.5 and 6.25 μg·m L^（-1）） were prepared to act on HepG_2 cells, real time cellular analysis was used to record 96 h cell index（CI） to observe the effect of Myricanol and 5FEM on the proliferation and migration of HepG_2 cells. RESULTS Myricanol and 5FEM could inhibit the proliferation and migration of HepG_2 cells, showing a dose-effect curve. The effect of 5 FEM was better than myricanol at the same dose. CONCLUSION Myricanol and 5FEM can effectively inhibit the proliferation and migration of HepG_2 cells.

关 键 词：杨梅醇 5-对氟苄氧基杨梅醇 人肝癌HEPG2细胞 细胞增殖 细胞迁移 

分 类 号：R285.4[医药卫生—中药学]