干旱胁迫下转PEPC＋PPDK基因增强水稻光合机构的保护能力  

Under Drought Stress, the Protective Capability of Photosynthetic Apparatus of Transgenic PEPC+PPDK Rice was Enhanced

作　　者：姜蓓蓓 欧阳宁 孙学武[2] 谭炎宁[2] 孙志忠[2,3] 余东 辛世文[4] 段美娟 袁定阳[1,2,3] Jiang Beibei1,2, Ouyang Ning 1,2, Sun Xuewu 2, Tan Yanning 2 ,Sun Zhizhong 2,3 ,Yu Dong 2,3 ,Xin Shiwen 4, Duan Meijuan 2,3, Yuan Dingyang 1,2,3(1 State Key Laboratory of Hybrid Rice, Long Ping Branch, Graduate School of Hunan University, Changsha, 410000; 2 Hunan Hybrid Rice Research Center, Changsha, 410000; 3 College of Biological Science and Technology, Hunan Agricultural University, Changsha, 410000; 4 The Chinese University of Hong Kong, Hong Kong, 999077)

机构地区：[1]湖南大学研究生院隆平分院杂交水稻国家重点实验,长沙410000 [2]湖南杂交水稻研究中心,长沙410000 [3]湖南农业大学生物科学技术学院,长沙410000 [4]香港中文大学,香港999077

出　　处：《分子植物育种》2018年第3期798-806,共9页Molecular Plant Breeding

基　　金：转基因重大专项(2016ZX08001-004); 国家自然科学基金(31501243)共同资助

摘　　要：随着全球的经济发展和人口膨胀,水资源短缺现象日趋严重,干旱已成为粮食生产的主要限制因素之一。C_4植物(如玉米)具有较高的光合速率以及较高的水分利用效率,利用转基因技术将C_4植物高光合及耐旱特征引入C_3作物(如水稻),被认为是解决水资源短缺与全球粮食需求不断增长矛盾的重要手段。本研究以转PEPC+PPDK双基因水稻及其非转基因对照为材料,通过PEG6000模拟干旱条件,研究干旱胁迫下叶片叶绿素含量、叶绿素荧光参数、C_4光合酶活性、叶片抗氧化酶活性等光合生理相关参数变化。结果表明:干旱胁迫下转PEPC+PPDK基因水稻叶绿素含量、C_4光合酶活性、抗氧化能力等均有提升;干旱胁迫对转PEPC+PPDK基因水稻PSⅡ的光化学活性抑制较小,对细胞的损伤相对更低,表明转基因水稻对光合机构的保护能力明显增强。With the global ec onomic development and expansion of the population, the shortage of water resources is becoming more and more serious, and the drought has become one of the main limiting factors of food production. Because the C_4 plant（such as the corn） has higher photosynthetic rate and higher water use efficiency,it is considered to be an important method to solve the contradiction of the shortage of water resources and the growing demand of the global food, that the main characteristic of high photosynthetic and drought tolerance in the C_4 plant is introduced into C_3 crop（such as the rice） through using the transgenic technology. In this study, the author uses the comparison of the PEPC ＋PPDK transgenic rice and non-transgenic of it as materials, simulate drought conditions through the PEG6000, and research the changes of chlorophyll content, chlorophyll fluorescence parameters, C_4 photosynthetic enzyme activities and antiox idant enzyme activities on the leaf. The results show that under the stress of the drought, the transgenic rice has been promoted in many respects, such as C_4 photosynthetic enzyme activity, chlorophyll content and antioxidant capacity and so on, that profits from PEPC＋PPDK＇s bi-gene, and under the common action of these factors, it has reduced the inhibition of PSII＇s photochemical activity on account of drought＇s stress, and it has reduced the damage on the cell because of the drought＇s stress. The results indicate that the capability of genetically modified rice in protecting the photosynthetic apparatus has been dramatically enhanced.

关 键 词：水稻 磷酸烯醇丙酮酸羧化酶 转基因 干旱胁迫 

分 类 号：S511[农业科学—作物学]