海南普通野生稻对水稻白叶枯病的抗性鉴定  

Identification of Resistance of Wild Rice in Hainan to Bacterial Blight of Rice

作　　者：李栋 何美丹 吴丹 赵建发 何光亮 夏志辉 袁潜华 Li Dong ,He Meidan ,Wu Dan, Zhao Jianfa ,He Guangliang, Xia Zhihui,Yuan Qianhua(Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228)

机构地区：[1]海南大学热带农林学院,海口570228

出　　处：《分子植物育种》2018年第3期832-839,共8页Molecular Plant Breeding

基　　金：海南耕地改良关键技术研究与示范专项项目(HNGDg12015);公益性行业(农业)科研专项经费项目(201403-075)共同资助

摘　　要：白叶枯病严重危害水稻的生产,防治白叶枯病的工作刻不容缓。目前最有效的防治措施是培育具有抗性的水稻新品种。但是来源于栽培稻的抗病基因抗谱窄、数量有限,因此从野生稻中发掘抗白叶枯病基因,对培育具有广谱抗性的水稻新品种具有重要意义。本研究通过接种4个菲律宾白叶枯病菌小种对海南普通野生稻进行抗性鉴定,同时利用目前克隆的已知抗性基因设计分子标记进行PCR分析,检测了其中的抗白叶枯病基因。结果表明,来自海南不同地区的15个普通野生稻居群均表现出对白叶枯病菲律宾小种一定的抗性。功能标记检测结果发现,15个居群的普通野生稻中含有Xa1或其同源基因,但均不含有xa5、xa13抗病基因;7个居群能够扩增出Xa21抗病基因条带;Xa27抗病基因条带能够在另外7个居群中被扩增出。研究结果为发掘具有抗白叶枯病特性的海南普通野生稻材料提供了新的理论参考依据。Bacterial blight（BB） seriously harms the production of rice, and the work of preventing and controlling bacterial blight is urgent. At present, the most effective prevention and cure measure is to breed new rice varieties with resistance. However, the resistance genes of rice derived from cultivated rice are narrow and limited in quantity, so it is important to explore the gene of resistance to bacterial blight from wild rice for breeding new varieties with broad spectrum resistance. In this study, the resistance of common wild rice in Hainan was evaluated by inoculating with 4 Philippine races of BB. At the same time, we designed the molecular markers with the known resistance genes, and analyzed the anti-bacterial blight genes by PCR. The results showed that 15 wild rice varieties from different parts of Hainan had certain resistance to Philippine races of BB. According to functional marker detection, 15 wild rice varieties contained Xa1 or its homologous gene, but all did not contain resistance genes xa5 and xa13. 7 populations were able to amplify Xa21 disease resistant gene bands. Xa27 resistant gene bands could be amplified in 7 other populations. The results of this study could provide a new theoretical basis for the discovery of common wild rice plants with resistance to bacterial blight in Hainan.

关 键 词：海南野生稻 白叶枯病 抗性基因 分子标记 

分 类 号：S435.111.47[农业科学—农业昆虫与害虫防治]