高效冬枣花药愈伤组织培养体系的建立  

Establishment of a High-efficiency Anther Callus Culture System of Dongzao(Ziziphus jujuba Mill.)

作　　者：贾际平 吴丽萍 曹明 孔德仓 李颖岳[1] 庞晓明[1,2] Jia Jiping 1,2, Wu Liping 1, Cao Ming 3 ,Kong Decang 3, Li Yingyue 1, Pang Xiaoming 1,2(1 National Engirteefing Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Ed- ucation, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083; 2 Key Laboratory of Urban Agriculture （North China, Ministry of Agriculture, Beijing, 102206; 3 National Station for Improved Cultivar of Jujube, Cangzhou, 061000)

机构地区：[1]北京林业大学生物科学与技术学院林木育种国家工程实验室林木花卉遗传育种教育部重点实验室,北京100083 [2]中华人民共和国农业部华北都市农业重点实验室,北京102206 [3]河北省沧县国家枣树良种基地,沧州061000

出　　处：《分子植物育种》2018年第3期948-953,共6页Molecular Plant Breeding

基　　金：科技部“十二五”国家科技支撑计划项目(2013BAD14B0302); 北京农学院农业部华北都市农业重点实验室开放课题(kf2017015); 国家自然科学基金项目(31372019)共同资助

摘　　要：以冬枣花药为试验材料,研究影响愈伤组织诱导、增殖和分化的因素,优化各因素建立高效的冬枣花药培养体系。结果表明:在愈伤组织诱导过程中,加入0.4~0.6 mol/L的甘露醇4℃处理1~3 d可提高愈伤组织的诱导率。最适的愈伤组织诱导培养基为:MS+1.0 mg/L 2,4-D+0.5 mg/L TDZ+30 g/L麦芽糖,诱导率可达到69.72%。愈伤组织增殖的最适培养基为MS+0.3 mg/LNAA+1.0 mg/LTDZ+30 g/L麦芽糖;在WPM+0.2 mg/L NAA+0.5 mg/L TDZ+20 g/L麦芽糖时,分化率最高可达89.00%。An efficient callus culture system was established using anther of Dongzao（ Ziziphus jujuba Mill.）.Different influencing factors of callus induction, proliferation and differentiation of the anthers were investigated in this study. The results showed that the adding 0.4-0.6 mol/L mannitol at 4℃ for 1-3 days could increase callus induction rate in the callus induction process. The optimum callus induction medium was MS ＋1.0 mg/L 2,4-D＋0.5 mg/L TDZ ＋30 g/L maltose, which resulted in induction rate as high as 69.72%. The medium of callus proliferation was MS ＋0.3 mg/L NAA ＋1.0 mg/L TDZ ＋30 g/L maltose. The differentiation rate could be up to89.00% under WPM＋0.2 mg/L NAA＋0.5 mg/L TDZ＋20 g/L maltose.

关 键 词：冬枣(Ziziphus JUJUBA Mill.) 花药 愈伤组织 诱导 分化 

分 类 号：S665.1[农业科学—果树学]