H_2O_2对杜仲愈伤组织中黄酮含量及过氧化物酶活性的影响  

Effects of Hydrogen Peroxide(H_2O_2) on Flavonoid Content and Peroxidase Activity in Callus of Eucommia ulmoides Oliv.

作　　者：邓云云 张俊娥[1] Deng Yunyun ,Zhang Jun＇e(College of Life Science, Jiangxi Normal University, Nanchang, 330022)

机构地区：[1]江西师范大学生命科学学院,南昌330022

出　　处：《分子植物育种》2018年第3期1007-1010,共4页Molecular Plant Breeding

基　　金：国家自然科学基金(31100396);江西省科技厅自然科学基金面上项目(20171BAB204004);江西省教育厅科学技术研究项目(GJJ150332)共同资助

摘　　要：本研究分析了在加有不同浓度H_2O_2的B5培养基上生长的杜仲愈伤组织总黄酮含量变化及过氧化物酶(POD)活性的变化及其两者的相关性。结果表明,5~10 mmol/L H_2O_2培养杜仲愈伤组织10 d时总黄酮含量最高。当杜仲愈伤组织培养5~10 d、外源H_2O_2浓度为10 mmol/L时的POD活性最高。相关性分析表明,杜仲愈伤组织总黄酮含量与POD活性之间的相关系数为r=0.420 61,具有一定的相关性,推测5~10 mmol/L H_2O_2可通过提高杜仲愈伤组织中POD酶活性,刺激黄酮的积累。本研究为杜仲黄酮的代谢调控及机制研究及在杜仲愈伤组织中获取高含量类黄酮的杜仲突变体提供科学依据。This study analyzed the changes of the flavonoid content and the peroxidase（POD） activity in the callus of Eucommia ulmoides cultured in B5 medium with different concentrations of H2O2 and their correlation. The results showed that the flavonoid content in the callus of Eucommia ulmoides was the highest with 5-10 mmol/L H2O2 for 10 d culture. The POD activity was the strongest when the callus were cultured for 5-10 d with 10 mmol/L H2O2. The correlation analysis indicated that the correlation coefficient was r =0.420 61 between the flavonoid content in callus and the POD activity, which implied the flavonoid content and the POD activity had a certain correlation. It was speculated that 5-10 mmol/L H2O2 stimulated the accumulation of flavonoids by improving the POD activity of the calli. This study provided the scientific basis for the study of metabolic regulation and mechanism of mutants of Eucommia ulmoides flavonoids and obtain high content of flavonoids in the callus.

关 键 词：H2O2 杜仲 愈伤组织 黄酮 过氧化物酶 

分 类 号：S567.19[农业科学—中草药栽培]