喜树碱-氟尿苷纳米颗粒对口腔鳞癌细胞Tca-8113增殖与迁移的影响  

The Influence of Camptothecin-fluorouridine Self-assembled Nanoparticles on the Proliferation and Migration of Oral Squamous Cell Carcinoma Tca-8113 Cells

作　　者：唐明智 沈龙飞 陈嘉彬 张倩 李国林[1] 单秋生 TANG Ming-zhi1, SHEN Long-fe, CHEN Jia-bin, ZHANG Qian, LI Guo-lin , SHAN Qiu-sheng(1 Department of Oral and Maxillofacial Surgery, AftTliated Stomatological Hospital, Harbin Medical University, Harbin, Heilongjiang, 150001, China; 2 Chongqing Three Gorges Medical College, Chongqing, 404120, Chin)

机构地区：[1]哈尔滨医科大学附属口腔医院口腔颌面外科,黑龙江哈尔滨150001 [2]重庆三峡医药高等专科学校,重庆404120

出　　处：《现代生物医学进展》2018年第2期230-234,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81272466)

摘　　要：目的:探究喜树碱-氟尿苷(CPT-FUDR)纳米颗粒对口腔鳞癌Tca-8113细胞增殖与迁移的影响。方法:制备喜树碱-氟尿苷纳米颗粒,通过丁达尔现象证明已组装完毕。将制备好的纳米颗粒组和喜树碱(CPT)单药组、氟尿苷(FUDR)单药组以及两种单药混合组(CPT/FUDR)作对比,采用MTT实验检测药物对口腔鳞癌细胞Tca-8113增殖的抑制作用,通过划痕实验探究CPT-FUDR纳米颗粒和CPT/FUDR混合药物对细胞迁移能力的影响。结果:MTT结果显示:在药物浓度大于0.1μM时,随着浓度的增加,四组细胞存活率均明显下降(P<0.05),而CPT-FUDR纳米颗粒组Tca-8113细胞的存活率明显低于单药CPT、FUDR和CPT/FUDR混合物组(P<0.05)。在划痕实验中,培养48 h后,CPT/FUDR混合物组和CPT-FUDR纳米颗粒组均显著低于空白组(P<0.05),且CPT-FUDR纳米颗粒组显著低于CPT/FUDR混合物组(P<0.05)。结论:在体外,CPT-FUDR纳米颗粒对口腔鳞癌Tca-8113细胞的增殖与迁移有较好的抑制作用,且抑制效果优于CPT/FUDR两种单药混合。Objective： To explore the influence of camptothecin-fluorouridine（CPT-FUDR） self-assembled nanoparticles on the proliferation and migration of oral squamous cell carcinoma Tca-8113 cells. Methods： The camptothecin-fluorouridine self-assembled nanoparticles which could be confirmed by the Tyndall phenomenon were prepared. MTT was used to detect the inhibitory effect of drugs on the proliferation of oral squamous cell carcinoma cell line Tca-8113 by comparing the nanoparticles group, camptothecin prepared（CPT） single drug group, Floxuridine（FUDR） single drug group and two single drug mixed group（CPT/FUDR）. Wound scratch assay was used to explore the effect of nanoparticles and CPT/FUDR mixture of drugs on the cell migration of Tca-8113 cells. Results：MTT results showed that the cell viability of four groups were dramatically decreased（P0.05） as the increase of drug concentration.when the concentration of drug was higher than 0.1 μM, the viability of Tca-8113 cells in CPT-FUDR nanoparticles group was significantly lower than thoseof the CPT group, FUDR group and CPT/FUDR mixed group（P0.05）. Wound scratch assay showed that the healing rate of CPT/FUDR mixed group and CPT-FUDR nanoparticles group were dramatically lower than that of the control group（P0.05）, which was higher in the CPT-FUDR nanoparticles group than that of the CPT/FUDR mixed group（P0.05） after incubating for 48 h. Conclusion： In vitro, the CPT-FUDR nanoparticles had inhibitory effect on the the proliferation and migration of oral squamous cell carcinoma Tca-8113 cells, which was better than that of CPT/FUDR mixture.

关 键 词：口腔癌 联合化疗 口腔鳞癌 TCA-8113 

分 类 号：R33[医药卫生—人体生理学]