越鞠丸加味对反流性食管炎大鼠食管黏膜PCNA、p53、CyclinD1表达的影响  

Effects of Modified Yueju Wan(越鞠丸) on Expressions of PCNA,p53,CyclinD1 in Esophageal Mucosa of Reflux Esophagitis Rats

作　　者：郑婷婷[1] 叶蔚 叶斌[2] 刘彬彬[2] ZHENG Tingting1, YE Wei2 , YE Bin2 , LIU Binbin2(1Jinhua Municipal Central Hospital , J inhua , Zhejiang 321001 ; 2 Hangzhou Chinese Medicine Hospital, Hangzhou,Zhejiang 31000)

机构地区：[1]浙江省金华中心医院,浙江金华321000 [2]杭州市中医院,浙江杭州310007

出　　处：《中国中医药科技》2018年第2期184-187,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省中医药管理局资助项目(2008CB053;2009CA086);杭州市卫生局资助项目(2008B0062)

摘　　要：目的:观察越鞠丸加味对反流性食管炎大鼠食管黏膜PCNA、p53和CyclinD1表达的影响。方法:采用"不全幽门结扎+贲门肌切开术"法建立大鼠反流性食管炎模型。50只SD大鼠随机分为正常组、假手术组、模型组、越鞠丸加味组、奥美拉唑组,每组10只。各药物组连续给药3周后。HE染色观察大鼠食管黏膜病理学改变;免疫组化法检测PCNA、p53、CyclinD1的表达。结果:从大体上和病理上观察模型组大鼠RE评分与正常组组比较有明显差异(P<0.01);两药物组评分较模型组明显降低(P<0.01)。模型组大鼠食管黏膜PCNA、p53、CyclinD1表达较正常组、假手术组显著增高(P<0.01);越鞠丸加味组和奥美拉唑组大鼠食管黏膜PCNA、p53、CyclinD1表达较模型组显著降低(P<0.01);越鞠丸加味治疗组PCNA、CyclinD1表达低于奥美拉唑组,p53表达两组之间差异无显著性。结论:越鞠丸加味能明显改善反流性食管炎大鼠食管黏膜的病理学改变,减轻食管炎症,降低RE大鼠食管黏膜PCNA、p53、CyclinD1的表达可能是其治疗RE的作用机理之一。Objective： To observe the effects of the Modified Yueju Wan on the expressions of PCNA, p53, CyclinD1 in esophageal mucosa of reflux esophagitis rats. Methods： The model of reflux esophagitis rat was built by ＂incomplete pyloric ligation ＋ cardia myotomy＂. 50 SD rats were divided into 5 groups： the normal group, the sham - operated group, the model group, the Modified Yueju Wan group and the omeprazole group. After 3 weeks of continuous administration, the esophageal tissues were dyed by HE and observed by optical microscope, while the expressions of PCNA, p53, CyclinD1 were observed by immunohistochemistry. Results： The expres- sions of PCNA, p53, CyclinD1 in esophageal mucosa were significantly higher in reflux esophagitis group com- pared with the normal control group and shamoperated group; the expressions of PCNA , Cyclin D1 were significantly lower in Modified Yueju Wan group than those of omeprazole group ,the expression of p53 has no significant difference between the two groups. Conclusion： Modified Yueju Wan can reduce the expression of PC- NA, p53 and CyclinD1 in esophageal mucosa of the reflux esophagitis rats, which may be one of the mechanisms of action of the treatment of RE.

关 键 词：越鞠丸加味 反流性食管炎 PCNA p53 CyclinD1 大鼠 

分 类 号：R285.5[医药卫生—中药学]