人参皂甙Rg3对人肠癌细胞增殖、迁移能力的影响  

Effects of Ginsenoside Rg3 on Proliferation and Migration of Human Colon Cancer Cells

作　　者：王英 李刚[2] WANG Ying1, LI Gang2(1 Wenzhou People＇ s Hospital, Wenzhou, Zhejiang 325000 ; 2 Shiyan People＇ s Hospital, Shiyan, Hubei 44200)

机构地区：[1]温州市人民医院,浙江温州325000 [2]十堰市人民医院,湖北十堰442000

出　　处：《中国中医药科技》2018年第2期191-193,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省温州市科技计划项目(Y20140101)

摘　　要：目的:探讨不同浓度人参皂甙Rg3对人肠癌细胞增殖、迁移能力的影响。方法:选取人肠癌细胞株LOVO为研究对象,人参皂苷Rg3低、高剂量组向细胞培养基中分别加入不同终浓度的人参皂苷Rg3(20、40 mg/L),并以正常培养基的LOVO细胞为对照组。各组细胞培养96 h后采用CCK-8实验和Transwell实验分别检测各组细胞的增殖能力和迁移能力;Western blot检测E-cadherin蛋白表达情况。结果:与对照组相比,人参皂苷Rg3组LOVO细胞增殖能力均呈现不同程度的抑制(P<0.05),且随Rg3浓度的增加,其抑制能力增强;对照组、人参皂苷Rg3 20 mg/L和40 mg/L组穿膜细胞数分别为(135.6±24.8)个、(109.6±22.2)个和(56.1±16.3)个,人参皂苷Rg3组穿膜细胞数显著低于对照组(P<0.05),且随Rg3剂量增高,穿膜细胞数逐渐降低;对照组、人参皂苷Rg3 20 mg/L和40 mg/L组LOVO细胞Ecadherin蛋白相对表达量分别为(1.0±0.21)、(6.56±1.23)和(8.78±1.56),人参皂苷组显著高于对照组(P<0.05)。结论:人参皂甙Rg3在体外对肠癌细胞LOVO增殖具有抑制作用,且存在一定的剂量效应关系,其对LOVO细胞迁移的抑制可能与上调细胞E-cadherin蛋白表达有关。Objective： To investigate the effects of Ginsenoside Rg3 on proliferation and migration of human eoloreetal eaneer cells. Methods： Human colon cancer cell line LOVO was selected as the research object, Ginsenoside Rg3 low and high dose groups were added into the cell culture medium with different concentrations of Ginsenoside Rg3 （20, 40 mg/L）, and the normal medium LOVO cells were used as the control group. After 96 h of cell cultured, the proliferation and migration ability of each group were detected by CCK - 8 test and Tran- swell test, and the expression of E - cadherin protein was detected by Western blot. Results： Compared with the control group, the proliferation ability of LOVO cells in Ginsenoside Rg3 group was inhibited in different degrees （ P 〈 0.05 ）, and the inhibition ability was enhanced with the increasing of Rg3 concentration. The trans mem- brane cells of control group, Ginsenoside Rg3 20 mg/L and 40 mg/L groups were （ 135.6 ±24. 8 ） , （ 109. 6 ± 22. 2） and （56. 1± 16. 3 ）, eells number of Ginsenoside Rg3 groups were significantly lower than that of the eon- trol group （ P 〈 0.05 ） , and along with the increased of dose, the penetrating cells number gradually reduced ; E -eadherin protein expression of LOVO cells in control group, Ginsenoside Rg3 20 mg/L and 40 mg/L were （ 1.0±0. 21 ） , （6. 56 ±1.23 ） and （ 8.78 ±1.56 ） respectively, E - eadherin protein expression in Ginsen- oside Rg3 group was significantly higher than that of the control group （ P 〈 0. 05 ）. Conclusion： Ginsenoside Rg3 can inhibit the proliferation of colon eaneer cell line LOVO in vitro, and there is a dose effect relationship. The inhibition of LOVO eel1 migration may be related to the up - regulation of E - eadherin protein expression.

关 键 词：肠癌 人参皂甙RG3 增殖 迁移 体外实验 

分 类 号：R285[医药卫生—中药学]