矽尘致工人FOXP3 DNA甲基化改变的研究  

Study of FOXP3 DNA methylation on workers exposed to silica dust

作　　者：廖静[1] 李小峰[2] 王春香 LIAO Jing1, LI Xiao-feng2, WANG Chun-xiang3(1.Occupational Disease Prevention and Control Department,Shenzhen Longgang District Center for Disease Control and Prevention,Shenzhen Guangdong,518172,China;2.Clinical Laboratory,Longgang District People＇s Hospital of Shenzhen, Shenzhen Guangdong, 518172,China;3.Occupational Health Department,Buff Health Care Center of Longgang District,Shenzhen Guangdong, 518112, Chin)

机构地区：[1]深圳市龙岗区疾病预防控制中心职业病防治科,广东深圳518172 [2]深圳市龙岗区人民医院检验科,广东深圳518172 [3]深圳市龙岗区布吉预防保健所职业卫生科,广东深圳518112

出　　处：《职业与健康》2018年第2期162-166,共5页Occupation and Health

基　　金：深圳市科技计划项目(JCYJ20150402164314696);深圳市龙岗区科技发展资金(YLWS20150513174008294);深圳市龙岗区科技发展资金(YLWS20150511114456591);深圳市卫生计生系统科研项目(201402126)

摘　　要：目的了解矽尘对作业工人叉头转录因子3(forkhead/winged helix transcription factor,FOXP3)基因启动子区域DNA甲基化状态的影响。方法选取健康检查者和矽尘作业工人为对照组和接尘组,采用亚硫酸氢盐测序法检测外周血FOXP3基因启动子特定区域DNA甲基化水平。结果在男性中,无论对照组还是接尘组FOXP3基因启动子区(+2071,+2182)5个Cp G位点均呈现高甲基化(100%)。在女性中,对照组第1个Cp G位点(+2102)和第4个Cp G位点(+2144)呈现低甲基化(40%),而接尘组相关Cp G位点可见高甲基化(100%)。结论女性接尘作业工人FOXP3基因启动子特定位点DNA高甲基化可能与矽尘的毒性有关。[Objective]To investigate the effect of silica dust on DNA methylation in the promoter of transcription factor forkhead box protein 3（FOXP3） gene in the exposed workers.[Methods] Then the level of DNA methylation in the particular region of the FOXP3 gene promoter was in the peripheral blood detected by bisulfite sequencing.[Results] In males,hypermethylation（100%）was observed in 5 Cp G sites of FOXP3 gene promoter（＋2071,＋2182） in the control group as well as in the exposure group.In females,the first Cp G site（＋2102）and the fourth Cp G site（＋2144）were found to be hypomethylated（40%） in the control group,while those in the exposure group were hypermethylated（100%）.[Conclusion] The hypermethylation in the particular sites of the FOXP3 gene promoter might be relevant with the toxicity induced by silica dust in the female workers.

关 键 词：职业病 FOXP3基因 DNA甲基化 矽尘 

分 类 号：R135.2[医药卫生—劳动卫生]