百合抗病无性系抗枯萎病的蛋白质组学分析  

Proteomics Analysis of Lily Blight Disease-resistant Clones

作　　者：张艺萍 瞿素萍[2] 杨秀梅[2] 马璐琳[2] 许凤[2] 王继华[2] 何月秋[1] ZHANG Yiping1,2, QU Suping2, YANG Xiumei2, MA Lulin2, XU Feng2, WANG Jihua 2 , and HE Yueqiu1(1College of Plant Protection, Yunnan Agricultural University, Kunming 650205, China; 2 Flower Research Institute, Yunnan Academy of Agricultural Sciences, National Engineering Research Center for Ornamental Horticulture, Yunnan Flower Breeding Key Laboratory, Yunnan Flower Engineering Center, Kunming 650205, Chin)

机构地区：[1]云南农业大学植物保护学院,昆明650205 [2]云南省农业科学院花卉研究所,国家观赏园艺工程研究中心,云南省花卉育种重点实验室,云南省花卉工程技术研究中心,昆明650205

出　　处：《园艺学报》2018年第3期530-540,共11页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31260484); 云南省科技人才和平台计划项目(2017HB083); 云南省科技创新人才计划--科技领军人才项目(2016HA005); 国家高技术研究发展计划“863”项目(2011AA100208)

摘　　要：以百合抗病无性系和感病无性系为试验材料,开展了百合抗尖孢镰刀菌枯萎病的蛋白质组学研究,以期明确抗病无性系参与抗病反应的关键蛋白。利用蛋白质双向凝胶电泳技术,对被百合尖孢镰刀菌诱导0、24、48和72 h的抗病无性系和感病无性系进行差异蛋白分析。经质谱检测及数据库检索,鉴定了25个差异蛋白质点,其中10个鉴定为未知功能的蛋白,其余15个依据其相关功能分为4类,即防御反应相关蛋白、光合作用相关蛋白、糖类及能量代谢相关蛋白和热激蛋白。其中参与防御反应的病程相关蛋白(推定的抗病蛋白RPS2、推定的抗晚疫病同族蛋白R1C-3、过氧化物酶Q、抗坏血酸过氧化物酶和NBS-LRR类似蛋白)可能是百合抗病无性系介入抗病防御反应的关键蛋白。To elucidate the key proteins related to disease-resistance of lily induced by Fusarium oxysporum f. sp. lilii,protein two-dimensional gel electrophoresis technology was used to analyze profiles of the disease-resistant and susceptible clones after inoculation of Fusarium oxysporum f. sp. lilii for 0,24,48 and 72 h. Tweenty-five differentially expressed proteins were successfully identified,including 10 differentially expressed proteins identified as unknown function proteins. The rest of the 15 differentially expressed proteins can be categorized into 4 classes according to the function,namely the defense reaction,photosynthesis,carbohydrate and energy metabolism,and heat shock-related proteins. The disease related proteins participated in the defense reaction including presumption of disease-resistant protein RPS2,putative late blight resistant protein homolog R1 C-3,peroxidase Q,ascorbate peroxidase and NBS-LRR-like protein,which may play key roles in the resistance against lily blight.

关 键 词：百合 总蛋白提取 双向电泳 尖孢镰刀菌百合专化型 差异蛋白质组 

分 类 号：S682.2[农业科学—观赏园艺]