胡萝卜始花期、主薹高和单株种子量的QTL分析  

QTL Analysis of Date of Initial Flowering,Main Stalk Length and Seed Weight per Plant in Carrot

作　　者：申琪 欧承刚[1] 孙婷婷[1] 刘波[1] 曹琼文 赵志伟[1] 孔小平 庄飞云[1,2] SHEN Qi1, OU Chenggang1, SUN Tingting1, LIU Bo1, CAO Qiongwen1, ZHAO Zhiwei1, KONG Xiaoping2, and ZHUANG Feiyun1,2(1The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, Ministry of Agriculture, Beijing 100081, China： 2Institue of Xining Vegetables, Xining 810000, Chin)

机构地区：[1]中国农业科学院蔬菜花卉研究所,农业部园艺作物生物学与种质创制重点实验室,北京100081 [2]西宁市蔬菜研究所,西宁810000

出　　处：《园艺学报》2018年第3期571-578,共8页Acta Horticulturae Sinica

基　　金：国家重大研发计划项目(2016YFD0100204); 国家现代农业产业技术体系建设专项资金项目(CARS-25); 中国农业科学院创新工程项目(CAAS-ASTIP-IVFCAAS); 青海省重点研发与转化计划项目(2017-NK-150)

摘　　要：以‘松滋野生’胡萝卜和橘色栽培品种‘Amsterdam’构建的回交重组自交系为试材,深入分析‘松滋野生’染色体片段对后代植株始花期、主薹高及单株种子量的遗传影响。试验结果表明,始花期在两年间均为偏正态分布,而主薹高均呈正态分布,单株种子量仅在2015年表现为正态分布。方差分析表明,3个性状在基因型间、年份间、基因型×年份间存在极显著差异。基于最新胡萝卜基因组数据构建了一张含有2 027个Bin(共154 776个SNP标记),总图距为1 436.433 c M,平均图距0.709 c M的遗传图谱。QTL定位分别获得了2、4、2个与始花期、主薹高和单株种子量相关位点,表型贡献率在14.6%~23.8%之间,其中始花期位点DIF5-1和主薹高位点MSL5-1位置重叠。A set of backcross inbred lines（BILs）crossed by a wild carrot species‘Songzi＇with an orange cultivar‘Amsterdam＇were used to deeply analyze the genetic influence of‘Songzi＇chromosome fragments on date of initial flowering（DIF）,main stalk length（MSL）,and seed weight per plant（SWP）. The experimental results suggested that MSL was normal distributed in 2014 and 2015,but DIF was on the contrary,and that SWP was only normal distributed in 2015. ANOVA analysis indicated that there was significant difference among BILs genotypes,years,and genotypes × years. Based on the last carrot genome resequencing data,a genetic map consisted of 2 027 Bins and 154 776 SNPs was developed,and the total genetic distance was 1 436.433 cM with an average interval of 0.709 cM between Bin markers. Two,four and two QTLs associated with DIF,MSL and SWP were discovered,respectively,with 14.6%–23.8% phenotypic variance. DIF5-1 locus was overlapped with MSL5-1 locus.

关 键 词：胡萝卜 回交重组自交系 始花期 主薹高 种子量 QTL 

分 类 号：S631.2[农业科学—蔬菜学]