利用黄秋葵转录组信息挖掘SSR标记及用于种质分析  

SSR Markers Excavation and Germplasm Analysis Using the Transcriptome Information of Hibiscus esculentus

作　　者：李永平[1] 刘建汀 陈敏氡[1] 张前荣[1] 朱海生[1] 温庆放[1] LI Yongping, LIU Jianting, CHEN Mindong, ZHANG Qianrong, ZHU Haisheng,WEN Qingfang(Crops Research Institute, Fujian Academy of Agricultural Sciences~ Vegetable Research Center, Fujian Academy of Agricultural Sciences： Fujian Engineering Research Center for Vegetables, Fuzhou 350013, Chin)

机构地区：[1]福建省农业科学院作物研究所/福建省农业科学院蔬菜研究中心/福建省蔬菜工程技术研究中心,福州350013

出　　处：《园艺学报》2018年第3期579-590,共12页Acta Horticulturae Sinica

基　　金：福建省属公益类科研院所基本科研专项(2015R1026-9);福建省农业科学院蔬菜科技创新团队项目(STIT2017-1-2);福建省农业科学院"青年科技英才百人计划"项目(YC2017-5);国家特色蔬菜产业技术体系项目(CARS-24-G-07)

摘　　要：通过对黄秋葵(Hibiscus esculentus L.)转录组信息分析,共获得74 600条unigene,全部序列有52 976 011 bp;有3 191条unigene包含SSR的序列,从中鉴定出3 448个SSR位点,其中有235条序列包含1个以上SSR,复合SSR有123个。优势重复基序为三核苷酸和二核苷酸,分别占总SSR的60.79%和21.43%。二核苷酸重复基元中以AG/CT为优势重复基元,占总位点的11.02%,三核苷酸重复基元以AGA/TCT为主,占总位点的19.61%。利用Primer 3.0共设计出8 088对SSR引物。从87对有效扩增引物中随机选择60对用于32份黄秋葵种质的多态性验证分析,其中36对(占60%)引物表现稳定可重复的多态性。利用UPGMA作图,将32份供试材料分为2类。利用黄秋葵转录组数据进行SSR标记开发,能获得较高频率的SSR位点,且类型丰富,为其遗传多样性分析和遗传图谱构建提供更丰富可靠的标记选择。A total of 74 600 unigenes were obtained by transcriptome sequencing analysis from Hibiscus esculentus. The whole sequence was 52 976 011 bp and a total of 3 448 SSR loci were identified from 3 191 unigenes. 235 sequences contained more than one SSR locus and compound SSR loci sequences were 123. The major types such as trinucleotide and dinucleotide were accounted for 60.79% and 21.43%,respectively. Furthermore,the AG/CT was the predominant dinucleotide repeat type（11.02%）,and the AGA/TCT was the predominant trinucleotide repeat type（19.61%）. 8 088 pairs of SSR primers were found by Primer 3.0 and 60 pairs of primers were randomly selected from 87 pairs of effective amplification primers for polymorphism analysis of 32 Hibiscus esculentus samples. 36 primers（60%）showed stable and repeatable polymorphism. According to the UPGMA analysis,32 samples were divided into 2 groups. These results indicated that high frequency and various types of SSR markers could be acquired in Hibiscus esculentus by transcriptome sequencing analysis and abundant SSR markers would provide more reliable markers for genetic diversity analysis and genetic mapping construction in Hibiscus esculentus.

关 键 词：黄秋葵 转录组 SSR 多态性 

分 类 号：S649[农业科学—蔬菜学]