二萜类化合物蓝萼甲素(glaucocalyxin A)对拟南芥四种磷脂酶的影响  

Effects of glaucocalyxin A on four phospholipases in Arabidopsis thaliana

作　　者：杨鹏军[1] 陈璐[1] 仇奕之 安炎黄 赵敏[1] 杨宁[1] YANG Peng-jun, CHEN Lu, QIU Yi-zhi, AN Yan-huang, ZHAO Min, YANG Ning(College of Life Science, Northwest Normal University, Lanzhou 730070, China)

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《生态学杂志》2018年第4期1058-1064,共7页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(31360061和31660116)资助

摘　　要：为了探究二萜类化合物蓝萼甲素(glaucocalyxin A)对拟南芥4种磷脂酶的影响,以蓝萼甲素为供体,以哥伦比亚野生型拟南芥(Col-0)和4个拟南芥磷脂酶突变体株系(pla1-1、pla2-1、plc1-1和pldδ-2)为受体植物,通过施加不同浓度的蓝萼甲素(0、20、100μmol·L-1)对5个拟南芥株系组织膜透性和渗透调节物质进行研究。结果表明:外施不同浓度的蓝萼甲素后,拟南芥5个株系中相对电导率和丙二醛(MDA)含量均显著升高,脯氨酸和可溶性蛋白含量呈现出先上升后下降的趋势,于24 h达到峰值,且不同的磷脂酶在调控其生理过程中具有不同的作用;表明蓝萼甲素作用与4种磷脂酶具有相关性,可通过调控4种磷脂酶来发挥部分化感作用。The effects of different concentrations of diterpenoids glaucocalyxin A （0, 20, 100 μmol·L-1） on membrane permeability and osmotic adjustment of fiveArabidopsis thaliana lines （the ColombiaArabidopsis thaliana, and pla1-1, pla2-1, plc1-1 and pldδ2 phospholipase mutants） were examined. The results showed that the relative electrolyte leakage and malondialdehyde （MDA） contents were significantly increased by the application of glaucocalyxin A in five lines of A. thaliana. The contents of proline and soluble protein first increased and thendecreased, with a peak at 24 h. Different types of phospholipases played different roles in thephysiological regulation process. Our results suggest that the effects of glaucocalyxin A areassociated with the four types of phospholipids and that its allelopathic effects can be exerted byregulating phospholipases.

关 键 词：蓝萼甲素 拟南芥 磷脂酶 组织膜透性 渗透调节物质 

分 类 号：Q945[生物学—植物学]