千里光β-葡萄糖苷酶基因(ScBG1)的序列特征与表达分析  

Sequence Features and Transcription Expression Levels of β-Glucosidase Gene( ScBG1) in Senecio scandens

作　　者：钱倩[1] 钱秋博言 唐加 李林[3] 徐德林[3] 钱刚[3] QIAN Qian1, QIAN Qiu-bo-yan2 ,TANG Jia1, LI Lin3 ,XU De-lin3, QIAN Gang3(1. Department of Clinic Medicine, Zunyi Medical College, Zunyi 563003, China; 2. Qiannan Medical College for Nationalities, Duyun 558003, China ; 3. Department of Cell Biology,Zunyi Medical College, Zunyi 563003, Chin)

机构地区：[1]遵义医学院临床医学系,贵州遵义563000 [2]黔南民族医学高等专科学校,贵州都匀558003 [3]遵义医学院医学细胞生物学教研室,贵州遵义563000

出　　处：《中药材》2017年第9期2036-2041,共6页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(31560087);贵州省大学生创新创业训练计划项目(201610661016);遵义医学院大学生创新项目([2015]5021)

摘　　要：目的:探讨千里光β-葡萄糖苷酶(β-glucosidase,BG)基因(ScBG1)的序列特征及表达与抗菌性状的关系。方法:利用生物信息学软件分析千里光BG蛋白的序列特征、结构域,通过实时荧光定量PCR法(qRT-PCR)检测ScBG1基因在不同抗菌性状的亲本及其杂交F1代的转录累积水平。结果:千里光BG蛋白一级结构由459个氨基酸残基构成,C端保守基元序列是该蛋白的功能结构域;亚细胞定位分析结果显示BG蛋白分布于细胞质膜、线粒体膜和线粒体膜间腔;ScBG1基因转录累积水平与抗菌性状呈正相关。结论:千里光β-葡萄糖苷酶催化产物参与抗菌性状的次生代谢信号传导网络,其抗菌生理功能表现为核基因组与线粒体基因组共同控制的数量性状。Objective： To explore physiological role of β-glucosidase enzyme gene（ ScBG1） in Senecio scandens,and to clarify relationship between the structural domains and the transcript expression levels of ScBG1 gene and antibacterial characteristics. Methods：Bioinformatics software was applied to analyze the traits of β-glucosidase enzyme in the research. Moreover,quantitative real-time PCR（ qRT-PCR） was applied to detect the transcript expression levels of ScBG1 gene among the diverse individuals involving significant differential phenotypic parents and their hybrid F1 generations. Results： Primary structure included 459 amino acid residues in BG protein,which was further contributed to the formation of structural domains composed of C-terminal conserved sequence motif（ CSM）. Furthermore,investigation of sub-cellular localization indicated that ScBG1 protein mostly had a widespread distribution including cytoplasm membrane,mitochondrial membrane and mitochondrial membrane cavity. In addition,the positive correlation appeard in the transcript expression levels and the antibacterial characteristics among diverse individuals. Conclusion： The catalytic product of ScBG1 protein might play an important role in the secondary metabolism signal networks,associated with the antibacterial characteristics. The results also support the view that both nuclear genome and mitochondrial genome are contributed to antibacterial characteristics in Senecio scandens.

关 键 词：千里光 Β-葡萄糖苷酶基因 生物信息学 正反交 实时荧光定量PCR 

分 类 号：R282.71[医药卫生—中药学]