鸡血藤水提物抗抑郁作用研究  

Antidepressive-like Effect of Aqueous Extract from Spatholobus suberectus

作　　者：曹斌[1] 李冬梅 韦桂宁[1] 苏启表 苏华[1] 何飞[1] 农志欢[1] CAO Bin1 ,LI Dong-mei1 ,WEI Gui-ning1 ,SU Qi-biao2 ,SU Hua1, HE Fei1, NONG Zhi-huan1(1. Guangxi Institute of Chinese Medicine ＆ Pharmaceutical Science, Nanning 530022, China ; 2. College of Health Science, Guangdong Pharmaceutical University, Guangzhou 510006, Chin)

机构地区：[1]广西中医药研究院,广西南宁530022 [2]广东药科大学健康学院,广东广州510006

出　　处：《中药材》2017年第9期2172-2176,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81360653;81560663);广东省中医药局建设中医药强省项目(20151270);广西中药质量标准研究重点实验室青年骨干创新基金(201606;201608)

摘　　要：目的:研究鸡血藤水提物的抗抑郁作用,并对其相关机理进行分析。方法:采用经典的小鼠悬尾实验和强迫游泳实验,观察鸡血藤水提物的抗抑郁作用。通过去势结合慢性不可预知性温和刺激复制雌性大鼠抑郁症模型,并从神经炎症的角度探讨鸡血藤水提物的抗抑郁作用机理。结果:鸡血藤水提物生药剂量8、4、2 g/kg均能显著缩短小鼠悬尾不动时间和游泳不动时间(P<0.05或P<0.01),而对小鼠自主活动无明显影响(P>0.05)。在抑郁大鼠实验中,鸡血藤水提物4 g/kg能显著缩短抑郁大鼠游泳不动时间(P<0.05)。鸡血藤水提物4、1 g/kg均能显著降低抑郁大鼠海马组织TNF-α和IL-1β水平(P<0.01),而对皮层组织TNF-α和IL-1β水平无明显影响(P>0.05)。免疫组化结果显示鸡血藤水提物4、1 g/kg能减少抑郁大鼠皮层和海马中NF-κBp65的表达。结论:鸡血藤水提物具有显著的抗抑郁作用,其作用机理可能与神经炎症的抑制有关。Objective： To investigate the antidepressive-like effect of aqueous extract from Spatholobus suberectus,and to study its related mechanism. Methods： The classical mice tail suspension test and mice force swimming test were used to evaluate the antidepressive-like effect of aqueous extract from Spatholobus suberectus. Female rat climacteric depression model was copied using the method of ovariectomy combined with chronic unpredictable mild stimulation（ CUMS）. The antidepressive-like mechanism of aqueous extract from Spatholobus suberectus was investigated from the view of nerve inflammation. Results： Aqueous extract from Spatholobus suberectus significantly decreased immobility time in tail suspension test and force swimming test in the doses of 8,4,and 2 g/kg（ P〈0. 05 or P〈0. 01）.However,it had no effects on autonomic activities. In depression rats experiment,aqueous extract of Spatholobus suberectus in the dose of4 g/kg significant decreased immobility time of depression rats in force swimming test（ P〈0. 05）. Aqueous extract from Spatholobus suberectus in the dose of 4,1 g/kg can significantly decreased the levels of TNF-α and IL-1β in hippocampus tissue of depression rats（ P〈0. 01）,while it has no obvious effects in cerebral cortex（ P〈0. 05）. Immunohistochemical results showed that the expression of NF-κBp65 in cerebral cortex and hippocampus tissue of depression rats were significantly decreased in the dose of 4,1 g/kg of aqueous extract of Spatholobus suberectus. Conclusion： Aqueous extract from Spatholobus suberectus has definitely anti depressive effect. The mechanism may be related to the inhibition of nerve inflammation.

关 键 词：鸡血藤水提物 抗抑郁 神经炎症 NF-ΚBP65 

分 类 号：R285.5[医药卫生—中药学]