固定化金属亲和层析分离纯化妥布霉素  

PURIFICATION OF TOBRAMYCIN BY IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHY

作　　者：牛猛 徐环昕[1,2] 宁方红 江邦和[1,2] 刘坐镇 NIU Meng1, XU Huanxin1,2 ,NING Fanghong1,2, JIANG Banghe1,2, LIU Zuozhen1,2(1. State Key Lab of Bioreactor Engineering, East China University of Science and Technology Shanghai 200237, China ;2. Huazhen Sci ＆ Tech Co. Ltd., Shanghai 200237, Chin)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]上海华震科技有限公司,上海200237

出　　处：《离子交换与吸附》2018年第1期74-85,共12页Ion Exchange and Adsorption

基　　金：上海市科学技术委员会项目(1402H248400)

摘　　要：通过研究4种不同金属配基的金属螯合亲和层析介质对妥布霉素的吸附和解吸性能,筛选出D401-Cu(Ⅱ)的分离纯化效果最好。通过静态吸附实验确定了最佳pH值为9.8,吸附动力学采用准二级动力学方程拟合较好,吸附等温线符合Langmuir吸附等温方程。通过动态吸附实验,确定最佳吸附条件:D401-Cu(Ⅱ)与D401的最佳配比为1:1,上样浓度为5.76mg/mL,上样流速为4BV/h;最佳洗脱条件为:分别使用浓度为0.3%和0.7%的氨水溶液进行梯度洗脱,洗脱剂用量均为6BV。采用优化后的工艺,妥布霉素纯度从32.85%提高到92.18%,回收率为97.46%,结果表明,D401-Cu(Ⅱ)能够用于妥布霉素的分离纯化。In this study, D401-Cu（II） with high performance of adsorption and desorption of tobramycin was selected from 4 different types of D401-M（Ⅱ） and establish the optimum technological conditions. In static state, the best pH value was 9.8, adsorption kinetics followed Pseudo-second-order rate equation. The adsorption equilibrium was well fitted by Langmuir formula. In dynamic state, the optimum adsorption conditions were specified as the flowings： the highest recovery ratio can be reached at the radio of 1 to 1 [D401-Cu（Ⅱ） to D401], the concentration of sample solution and flow rate were 5.76mg/mL and 4BV/h. The optimum desorption conditions was obtained by gradient elution with 0.3% and 0.7% Ammonia water, elution volume were 6BV, respectively. Under these conditions, tobramycin content were increased from 32.85% to 92.18%, with a tobramycin recovery rate of 97.46%. The results showed that D401-Cu（Ⅱ） could be used in separation and purification for tobramycin.

关 键 词：D401-Cu(Ⅱ) 妥布霉素 固定化金属亲和层析 纯化 

分 类 号：O647.3[理学—物理化学]