黄芪总苷对染镉大鼠细胞免疫功能及酶活性的影响  

Study of astragalosides on the cellular immunity and enzyme activity caused by cadmium in rats

作　　者：颜春鲁 安方玉 刘永琦 金华 伍志伟 苏韫 高军太 黄钰芳 YAN Chunlu1 , AN Fangyu1 , LIU Yongqi1 , JIN Hua1 , WU Zhiwei1 , SU Yun1 , GAO Juntai2, HUANG Yufang1(1. Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Study on Prevention and Treatment of Traditional Chinese Medicine, Gansu University of Traditional Chinese Medicine,Lanzhou, Gansu ,730000 P.R. China;2. Chinese Medicine Hospital of Pingliang , Pingliang , Gansu, 744000 P. R. Chin)

机构地区：[1]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,甘肃兰州730000 [2]平凉市中医医院,甘肃平凉744000

出　　处：《华西药学杂志》2018年第2期171-174,共4页West China Journal of Pharmaceutical Sciences

基　　金：甘肃省自然科学基金项目(1508RJZA046);甘肃中医药大学中青年基金项目(ZQ2014-9);甘肃中医药大学教学研究与教学改革项目(ZH-201606);甘肃省高等学校科研项目协同创新团队(2017C-14)

摘　　要：目的 探讨黄芪总苷对染镉大鼠免疫功能及酶活性的影响。方法 将36只大鼠随机均分为空白组、模型组、黄芪总苷组。末次给药24 h后处死大鼠,计算胸脾指数。MTT法测定脾淋巴细胞的转化功能和NK细胞的杀伤活性,比色分析法测定大鼠脾脏中SOD、MDA的活性及大鼠血清中AST、ALT、LDH的活性,用ELISA法测定大鼠脾脏组织中IL-2、TGF-β1的含量。结果 与空白组比较,模型组的体重、胸脾指数、SOD的活性、IL-2的含量、淋巴细胞转化能力和NK细胞杀伤能力均明显降低;但模型组MDA、TGF-β1的含量及AST、ALT、LDH的活性均明显升高。与模型组比较,黄芪总苷组的体重、脾脏指数、SOD的活性、IL-2的含量、淋巴细胞转化能力和NK细胞杀伤能力明显升高,而MDA、TGF-β1的含量及AST、ALT、LDH的活性均明显降低。结论 黄芪总苷对镉所致大鼠的酶活性和免疫损伤具保护作用。OBJECTIVE To study the effects of astragalosides on the immune function and enzyme activity in rats induced by cadmi- um. METHODS 36 SPF Wistar rats were randomly divided into three groups ： Blank group, cadmium - exposed group, and astragalo- sides group. After rats were sacrified, the indexes of thymus and spleen were calculated by weighing method. The transformation ability of T lymphocyte and the killing activity of NK cells were measured by MTT. The levels of SOD, AST, ALT, LDH and MDA were detected by colorimetrie assay. The contents of IL -2 and TGF -β1 were analyzed by ELISA. RESULTS Compared with the blank group, the weight and the indexes of thymus and spleen were decreased significantly in the cadmium - exposed group, the SOD activity and the IL- 2 content were also decreased significantly in the group. Meantime, the transformation ability of T lymphocyte and the killing activity of NK cells were reduced obviously in the cadmium - exposed group, while the levels of MDA,TGF - 131 , AST, ALT, LDH were increased. Compared with the cadmium - exposed group, the weights and the spleen index were both risen significantly in astragalosides treated group and the SOD activity and the IL -2 content were also improved significantly. The transformation ability of T lymphocyte and the killing activity of NK cells were enhanced obviously in astragalosides treated group. Yet the levels of MDA, TGF -β1, AST, ALT,LDH were lowered obviously in astragalosides treated group. CONCLUSION Astragalosides has protective effect on the immu- nity function damage and enzyme activity induced by cadmium in rats.

关 键 词：黄芪总苷 镉染毒 酶活性 免疫功能 淋巴细胞 NK细胞 保护作用 转化功能 杀伤能力 

分 类 号：R96[医药卫生—药理学]