疏肝健脾活血方对肝纤维化大鼠肝组织HIF-1α蛋白及VEGF mRNA表达的影响  

Effects of Shugan Jianpi Huoxue Decoction on hypoxia inducible factor-1α protein and vascular endothelial growth factor mRNA on liver fibrosis in rats

作　　者：夏雪皎[1] 林庚庭[2] 滕飞[1] 张俊杰[1] XIA Xue-jiao;LIN Geng-ting;TENG Fei;ZHANG Jun-jie(Zhejiang Chinese Medical University, Hangzhou 310053, China;The PLA 117 Hospital, Hangzhou 310004, China)

机构地区：[1]浙江中医药大学,杭州310053 [2]中国人民解放军第一一七医院,杭州310004

出　　处：《中华中医药杂志》2018年第4期1357-1360,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81473647);浙江省教育厅科研项目(No.Y201120995)~~

摘　　要：目的:应用二甲基亚硝胺(DMN)建立肝纤维化大鼠模型,动态观察疏肝健脾活血方对肝纤维化大鼠肝组织中HIF-1α蛋白、VEGF m RNA表达的影响,探讨疏肝健脾活血方抗肝纤维化的作用机制。方法:90只大鼠随机分为正常2、4、6周组,模型2、4、6周组,中药2、4、6周组,共9组,每组10只。采用DMN腹腔注射建立肝纤维化大鼠模型,同时中药各组给予疏肝健脾活血方灌胃。观察各组大鼠肝纤维化病理形态学改变,Westernblot法检测肝组织HIF-1α蛋白,RT-PCR法检测肝组织VEGF m RNA表达情况。结果:与正常各组同期比较,模型各组及中药各组大鼠肝组织HIF-1α蛋白、VEGF m RNA表达量均增高(P<0.01,P<0.05);与模型各组同期比较,中药4、6周组肝组织HIF-1α蛋白、VEGF m RNA表达量均降低(P<0.05,P<0.01)。结论:疏肝健脾活血方可减轻肝脏纤维化程度;降低肝组织HIF-1α蛋白、VEGF m RNA表达量,可能是其抗肝纤维化作用机制之一。Objective： To observe the effect of Shugan Jianpi Huoxue Decoction on HIF-lu protein and VEGF mRNA of liver fibrosis in rats induced by dimethylnitrosamine （DMN）, and study the mechanism of anti-hepatic fibrosis of Shugan Jianpi Huoxue Decoction. Methods： Ninety Wistar rats were divided randomly into nine groups, namely the normal groups （2w, 4w, 6w）, the model groups （2w, 4w, 6w）, the treatment groups （2w, 4w, 6w）, with ten rats in each groups. Excluding the normal group, other groups were injected DMN to set up liver fibrosis; the treatment group were given the Shugan Jianpi Huoxue Decoction by gavage at the same time. Each group was observed in rat liver histopathological changes, using Western blot and RT-PCR metheds to detect HIF-lu protein, VEGF mRNA expression of rat liver tissue. Results： Compared with the normal groups in the same period, the HIF-1α protein and VEGF mRNA expressions of hepatic tissue were enhanced in model groups and treatment groups （P〈0.01 P〈0.05）. Compared with the model groups in the same period, the HIF-1α protein and VEGF mRNA expressions of hepatic tissue of the treatment 4, 6w groups were decreased （P〈0.05, P〈0.01）. Conclusion： Shugan Jianpi Huoxue Decoction possesses a remarkable prophylactic effect on DMN induced liver fibrosis in rats, the mechanism may be related to reducing the protein and mRNA expressions of hepatic tissue HIF-1α, VEGF.

关 键 词：疏肝健脾活血方 二甲基亚硝胺 肝纤维化 缺氧诱导因子-1Α 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]