异常黏液质证候及阳痿病证模型大鼠睾丸间质细胞类固醇激素合成急性调节蛋白的表达  

The expression of steroidogenic acute regulatory protein in Leydig cells of abnormal phlegmatic syndrome and impotence rat model

作　　者：美合日古丽.萨塔尔 张盼盼 斯依提.阿木提 买买提祖农.买苏尔 姚巧玲 蒋萍 阿地力江.伊明 Meiheriguli Sataer1 , Zhang Panpan1 , Siyiti Amuti1 , Maimaitizunong Maisuer2 ,Yao Qiaoling3, JiangPing3 3, Adilijiang Yingming 1(1. Department of Human Anatomy, College of Basic Medicine, Xinjiang Medical University, Urumqi 830011, China; 2. Department of Pathophysiology, College of Basic Medicine, Xinjiang Medical University ; 3. Department of Physiology, College of Basic Medicine, Xinjiang Medical Universit)

机构地区：[1]新疆医科大学基础医学院人体解剖学教研室,乌鲁木齐830011 [2]新疆医科大学基础医学院病理生理学教研室 [3]新疆医科大学基础医学院生理学教研室

出　　处：《中国男科学杂志》2018年第1期3-7,共5页Chinese Journal of Andrology

基　　金：国家自然科学基金(项目编号:U1303322);新疆维吾尔自治区高校科研计划重点项目(项目编号:XJEDU2016I029)

摘　　要：目的探讨异常黏液质证候及阳痿病证模型大鼠睾丸间质细胞类固醇合成急性调节蛋白(StAR蛋白)的表达。方法建立异常黏液质证候模型,并筛选出阳痿病证模型后,分为证候模型组、病证模型组、证候用药组及病证用药组,两用药组采用伊木萨克片干预2周后,放免法检测各组血清中睾酮(T)含量,比较各组光镜及电镜下睾丸的形态学变化,免疫组织化学SP法及免疫印迹检测各组大鼠睾丸间质细胞StAR蛋白表达水平。结果 T含量检测结果显示,两模型组外周血清睾酮水平较正常组显著降低(P<0.05),证候用药组较证候模型组T水平显著上升,差异有统计学意义(P<0.05),病证用药组较病证模型组T水平亦有所上升,但差异无统计学意义(P>0.05)。睾丸形态学结果显示,光镜下正常组结构完好,两模型组可见生精细胞数量及层数明显减少,并伴生精上皮脱落,间质细胞数量减少;电镜下两模型组支持细胞核形不规则,胞质电子密度高,内质网扩张,部分生精细胞坏死,两用药组上述改变有所恢复。免疫组织化学结果显示,StAR在各组大鼠睾丸间质中均有表达,两模型组StAR表达水平显著低于正常组,差异有统计学意义(P<0.05);证候用药组StAR表达水平较证候模型组显著回升,而病证用药组StAR表达水平较病证模型组有所回升,但差异无统计学意义(P>0.05)。免疫印迹结果与免疫组织化学结果一致。结论异常黏液质证候及阳痿病证模型大鼠睾丸的形态结构发生病理改变可能与睾酮水平下降、睾丸间质细胞StAR表达下调有关。Objective To study the expression of steroidogenic acute regulator（StAR） protein in Leydig cells of rat model of abnormal phlegmatic syndrome and impotence disease. Methods We established rat model of abnormal phlegmatic syndrome and then screened for model of impotence disease. We divided the rats into normal group, abnormal phlegmatic syndrome group, impotence disease group, treatment group of abnormal phlegmatic syndrome and treatment group of impotence disease. After 2 weeks treatment with Yimusake, we detected the level of serum testosterone（T）, observed morphology of testicular tissue by optical microscope and electron microscope, and measured StAR expression in leydig cells by Immunohistochemistry and Western blot.Results The T levels of two model groups were significantly lower than that of normal group（P 〈 0.05）.Level of T in the treatment group of abnormal phlegmatic syndrome was increased obviously as compared with that of the syndrome model group（P 〈 0.05）.The T level in treatment group of impotence disease was also increased,but there was no significant difference（P 〈 0.05）.In abnormal phlegmatic syndrome group and impotence disease group,numbers and layers of sperm cells were significantly reduced,and accompanied by exfoliation of spermatogenic epithelium,and decrease of interstitial cells were also significant.Ultrastructure analysis showed that the nucleus of supporting cells was irregular,cell matrix density was darker,endoplasmic reticulum was expanded,and necrosistic permatagonial and spermatocytes cells were observed.While no significant structural changes were present in normal control group.After 2 weeks of treatment,minor restore was obtained in the two treatment groups;Immunohistochemical analysis showed the expression of StAR in cytoplasm of leydig cells in both model group was significantly lower than that in normal control group.The expression level of StAR in treatment group of abnormal phlegmatic syndrome was significantly higher than that in abnormal phlegma

关 键 词：异常黏液质 阳痿 睾酮 莱迪希细胞 

分 类 号：R277.5[医药卫生—中医学]